Overview

  • Product name
    Anti-NDUFB8 antibody [20E9DH10C12]
    See all NDUFB8 primary antibodies
  • Description
    Mouse monoclonal [20E9DH10C12] to NDUFB8
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Pig
  • Immunogen

    NDUFB8 from Cow

  • Positive control
    • Isolated mitochondria from Human heart, bovine heart, rat heart and mouse heart. Skeletal muscle tissue.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 22 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities
    Belongs to the complex I NDUFB8 subunit family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASHI antibody
    • CI-ASHI antibody
    • Complex I ASHI subunit antibody
    • Complex I-ASHI antibody
    • mitochondrial antibody
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial antibody
    • NADH-ubiquinone oxidoreductase ASHI subunit antibody
    • NDUB8_HUMAN antibody
    • NDUFB8 antibody
    see all

Images

  • All lanes : Anti-NDUFB8 antibody [20E9DH10C12] (ab110242) at 0.5 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from cow heart at 1 µg
    Lane 3 : Isolated mitochondria from rat heart at 10 µg
    Lane 4 : Isolated mitochondria from mouse heart at 10 µg

    Predicted band size: 22 kDa



    Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
  • Immunohistochemistry in serial sections of the muscle of patient AIV-5

    The patient is sufferening from Spastic paraplegia 7.

    Immunohistochemistry for complex I (NDUFB8) (A), complex II (B), complex III (C) and COX/SDH histochemistry (D) in serial sections of the muscle of patient AIV-5. There are complex I, III and IV deficient fibres, but complex I deficiency is most pronounced. Arrows mark serial sections of the same muscle fibers stained for different complexes.

    NDUFB8 (also refered to as complex I) was detected using ab110242 at 1/100 dilution).

    (After Figure 3 of Wedding et al)

  • Skeletal muscle immunohistochemistry using ab110242 on frozen tissue sections from a patient with a single large deletion of the mtDNA show a mosaic of complex I positive and complex I negative fibers.

References

This product has been referenced in:
  • Simon MT  et al. Novel mutations in the mitochondrial complex I assembly gene NDUFAF5 reveal heterogeneous phenotypes. Mol Genet Metab 126:53-63 (2019). Read more (PubMed: 30473481) »
  • Zhuang Y  et al. The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer. Cancer Metab 7:1 (2019). Read more (PubMed: 30697423) »
See all 92 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Human Tissue lysate - whole (Human brain lysate)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
10 µg
Specification
Human brain lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Sandrine Wauters

Verified customer

Submitted Apr 01 2016

Application
Western blot
Sample
Mouse Cell lysate - other (Mouse mitos from liver (A) and muscle (B))
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (3-12%)
Loading amount
10 µg
Specification
Mouse mitos from liver (A) and muscle (B)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 29 2015

Answer

Thank you for contacting us.

The MSDS for these products have been added to each product datasheet. They should appear on the website within the next few hours (time for the cache to be regenerated).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain- Cerebellum)
Specification
Brain- Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH8.0 EDTA
Permeabilization
No

Ms. Philippa Hepplewhite

Verified customer

Submitted Jun 28 2012

Answer

Thank you for your call earlier.

I now had a look at the homology between the immunogens of the antibodies included in the MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail (ab110413) and the respective proteins in Drosophila.

Unfortunately, the homologies are only between 34% and 76%. This is not enough to expect a cross-reaction of the antibody cocktail.

Therefore I cannot recommend ab110413 with drosophila samples.

I am sorry I did not have a positive answer to your question on this occasion and wish you good luck with your research.

Read More

Answer

Thank you for this additional information. I can confirm that ab110413 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail) contains 5 antibodies. The datasheets and more information about these antibodies can be found when following these links to their respective datasheets: ab14705: Click here (or use the following: https://www.abcam.com/index.html?datasheet=14705). ab14714: Click here (or use the following: https://www.abcam.com/index.html?datasheet=14714). ab14745: Click here (or use the following: https://www.abcam.com/index.html?datasheet=14745). ab14748: Click here (or use the following: https://www.abcam.com/index.html?datasheet=14748). ab110242: Click here (or use the following: https://www.abcam.com/index.html?datasheet=110242). As mentioned in my previous email all these antibodies have purified full-length proteins as immunogens and the actual binding site of the monoclonal antibodies was not further mapped. The purified proteins were extracted from cow (and human in case of ab14705). I am sorry I cannot provide more exact immunogen information on this occasion and hope this information was nevertheless helpful. Please do not hesitate to contact me with any further questions.

Read More

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