Product nameAnti-NDUFB8 antibody [20E9DH10C12]
See all NDUFB8 primary antibodies
DescriptionMouse monoclonal [20E9DH10C12] to NDUFB8
Tested applicationsSuitable for: IHC-P, WB, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human, Pig
NDUFB8 from Cow
- Isolated mitochondria from Human heart, bovine heart, rat heart and mouse heart. Skeletal muscle tissue.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
Purification notesNear homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Light chain typekappa
Our Abpromise guarantee covers the use of ab110242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 µg/ml. Predicted molecular weight: 22 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Sequence similaritiesBelongs to the complex I NDUFB8 subunit family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- ASHI antibody
- CI-ASHI antibody
- Complex I ASHI subunit antibody
All lanes : Anti-NDUFB8 antibody [20E9DH10C12] (ab110242) at 0.5 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from cow heart at 1 µg
Lane 3 : Isolated mitochondria from rat heart at 10 µg
Lane 4 : Isolated mitochondria from mouse heart at 10 µg
Predicted band size: 22 kDa
Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
Immunohistochemistry in serial sections of the muscle of patient AIV-5
The patient is sufferening from Spastic paraplegia 7.
Immunohistochemistry for complex I (NDUFB8) (A), complex II (B), complex III (C) and COX/SDH histochemistry (D) in serial sections of the muscle of patient AIV-5. There are complex I, III and IV deficient fibres, but complex I deficiency is most pronounced. Arrows mark serial sections of the same muscle fibers stained for different complexes.
NDUFB8 (also refered to as complex I) was detected using ab110242 at 1/100 dilution).
(After Figure 3 of Wedding et al)
Skeletal muscle immunohistochemistry using ab110242 on frozen tissue sections from a patient with a single large deletion of the mtDNA show a mosaic of complex I positive and complex I negative fibers.
This product has been referenced in:
- Simon MT et al. Novel mutations in the mitochondrial complex I assembly gene NDUFAF5 reveal heterogeneous phenotypes. Mol Genet Metab 126:53-63 (2019). Read more (PubMed: 30473481) »
- Kuang Y et al. Oxaloacetate induces apoptosis in HepG2 cells via inhibition of glycolysis. Cancer Med 7:1416-1429 (2018). Read more (PubMed: 29533007) »