Overview

  • Product name
    Anti-NDUFB8 antibody [20E9DH10C12]
    See all NDUFB8 primary antibodies
  • Description
    Mouse monoclonal [20E9DH10C12] to NDUFB8
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Pig
  • Immunogen

    NDUFB8 from Cow

  • Positive control
    • Isolated mitochondria from Human heart, bovine heart, rat heart and mouse heart. Skeletal muscle tissue.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 22 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities
    Belongs to the complex I NDUFB8 subunit family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASHI antibody
    • CI-ASHI antibody
    • Complex I ASHI subunit antibody
    • Complex I-ASHI antibody
    • mitochondrial antibody
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial antibody
    • NADH-ubiquinone oxidoreductase ASHI subunit antibody
    • NDUB8_HUMAN antibody
    • NDUFB8 antibody
    see all

Images

  • All lanes : Anti-NDUFB8 antibody [20E9DH10C12] (ab110242) at 0.5 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from cow heart at 1 µg
    Lane 3 : Isolated mitochondria from rat heart at 10 µg
    Lane 4 : Isolated mitochondria from mouse heart at 10 µg

    Predicted band size: 22 kDa



    Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
  • Immunohistochemistry in serial sections of the muscle of patient AIV-5

    The patient is sufferening from Spastic paraplegia 7.

    Immunohistochemistry for complex I (NDUFB8) (A), complex II (B), complex III (C) and COX/SDH histochemistry (D) in serial sections of the muscle of patient AIV-5. There are complex I, III and IV deficient fibres, but complex I deficiency is most pronounced. Arrows mark serial sections of the same muscle fibers stained for different complexes.

    NDUFB8 (also refered to as complex I) was detected using ab110242 at 1/100 dilution).

    (After Figure 3 of Wedding et al)

  • Skeletal muscle immunohistochemistry using ab110242 on frozen tissue sections from a patient with a single large deletion of the mtDNA show a mosaic of complex I positive and complex I negative fibers.

References

This product has been referenced in:
  • Simon MT  et al. Novel mutations in the mitochondrial complex I assembly gene NDUFAF5 reveal heterogeneous phenotypes. Mol Genet Metab 126:53-63 (2019). Read more (PubMed: 30473481) »
  • Kuang Y  et al. Oxaloacetate induces apoptosis in HepG2 cells via inhibition of glycolysis. Cancer Med 7:1416-1429 (2018). Read more (PubMed: 29533007) »
See all 89 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Human brain lysate)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
10 µg
Specification
Human brain lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Sandrine Wauters

Verified customer

Submitted Apr 01 2016

Application
Western blot
Sample
Mouse Cell lysate - other (Mouse mitos from liver (A) and muscle (B))
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (3-12%)
Loading amount
10 µg
Specification
Mouse mitos from liver (A) and muscle (B)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 29 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain- Cerebellum)
Specification
Brain- Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH8.0 EDTA
Permeabilization
No

Ms. Philippa Hepplewhite

Verified customer

Submitted Jun 28 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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