Overview

  • Product name

  • Description

    Rabbit polyclonal to NDUFB8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human NDUFB8 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab156057)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human Heart; Human Heart Mitonchondrial; Mouse Heart; Human Skeletal Muscle; Mouse Skeletal Muscle. This antibody gave a positive result in IHC in the following FFPE tissue: Human Heart muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab134367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 21 kDa).

Target

  • Function

    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities

    Belongs to the complex I NDUFB8 subunit family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ASHI antibody
    • CI-ASHI antibody
    • Complex I ASHI subunit antibody
    • Complex I-ASHI antibody
    • mitochondrial antibody
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial antibody
    • NADH-ubiquinone oxidoreductase ASHI subunit antibody
    • NDUB8_HUMAN antibody
    • NDUFB8 antibody
    see all

Images

  • IHC image of NDUFB8 staining in Human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134367, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-NDUFB8 antibody (ab134367) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human Heart Mitochondrial Lysate
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 5 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The band observed at 19 kDa could potentially be a cleaved form of NDUFB8 due to the presence of a 28 amino acid transit peptide. This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab134367 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab134367 has not yet been referenced specifically in any publications.

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