Overview

  • Product name

    Anti-NDUFB9 antibody [EPR15955-78]
    See all NDUFB9 primary antibodies
  • Description

    Rabbit monoclonal [EPR15955-78] to NDUFB9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human NDUFB9 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y6M9

  • Positive control

    • WB: Jurkat and HEK293 cell lysates; Human fetal liver lysate; Mouse kidney, mouse spleen, rat heart and rat spleen lysates. IHC-P: Human kidney and mouse cardiac muscle tissue. ICC/IF: Jurkat cells. Flow Cyt: Jurkat cells. IP: Jurkat whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15955-78
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab200198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
IP 1/150.
ICC/IF 1/250.
Flow Cyt 1/400.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities

    Belongs to the complex I LYR family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B22 antibody
    • CI B22 antibody
    • CI-B22 antibody
    • complex I B22 subunit antibody
    • Complex I-B22 antibody
    • DKFZp566O173 antibody
    • FLJ22885 antibody
    • I B22 antibody
    • LYR motif containing protein 3 antibody
    • LYR motif-containing protein 3 antibody
    • LYRM3 antibody
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 antibody
    • NADH ubiquinone oxidoreductase B22 subunit antibody
    • NADH-ubiquinone oxidoreductase B22 subunit antibody
    • NDUB9_HUMAN antibody
    • Ndufb9 antibody
    • UQOR22 antibody
    see all

Images

  • All lanes : Anti-NDUFB9 antibody [EPR15955-78] (ab200198) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate
    Lane 2 : HEK293 (Human embryonic kidney) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-NDUFB9 antibody [EPR15955-78] (ab200198) at 1/5000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-NDUFB9 antibody [EPR15955-78] (ab200198) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat heart lysate
    Lane 4 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB9 with ab200198 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasm staining on Human kidney tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling NDUFB9 with ab200198 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasm staining on mouse cardiac muscle tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NDUFB9 with ab200198 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Cytoplasm staining on Jurkat cell line is observed.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab200198 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NDUFB9 with ab200198 at 1/400 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • NDUFB9 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200198 at 1/150 dilution.

    Western blot was performed from the immunoprecipitate using ab200198 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Jurkat whole cell lysate 10 µg (Input).

    Lane 2: ab200198 IP in Jurkat whole cell elysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200198 in Jurkat whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

ab200198 has not yet been referenced specifically in any publications.

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