Product nameAnti-NDUFB9 antibody [EPR15955]
See all NDUFB9 primary antibodies
DescriptionRabbit monoclonal [EPR15955] to NDUFB9
Tested applicationsSuitable for: IHC-P, WB, Flow Cyt, ICC/IF, IPmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment within Human NDUFB9 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q9Y6M9
- Human fetal liver, HeLa, 293 and Jurkat lysates; Human kidney tissue; 293 and HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab188581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Sequence similaritiesBelongs to the complex I LYR family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- B22 antibody
- CI B22 antibody
- CI-B22 antibody
All lanes : Anti-NDUFB9 antibody [EPR15955] (ab188581) at 1/10000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB9 with ab188581 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling NDUFB9 with ab188581 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling NDUFB9 with ab188581 at 1/440 dilution compared to a Rabbit monoclonal IgG isotype control, followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot analysis of NDUFB9 immunoprecipitated from Human fetal liver lysate using ab188581 at 1/50 (Lane 1). Lane 2: PBS instead of Human fetal liver lysates.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
ab188581 has not yet been referenced specifically in any publications.