Overview

  • Product name

    Anti-NDUFB9 antibody [EPR15955]
    See all NDUFB9 primary antibodies
  • Description

    Rabbit monoclonal [EPR15955] to NDUFB9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment within Human NDUFB9 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y6M9

  • Positive control

    • Human fetal liver, HeLa, 293 and Jurkat lysates; Human kidney tissue; 293 and HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15955
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa).
Flow Cyt 1/440.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100.
IP 1/50.

Target

  • Function

    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities

    Belongs to the complex I LYR family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B22 antibody
    • CI B22 antibody
    • CI-B22 antibody
    • complex I B22 subunit antibody
    • Complex I-B22 antibody
    • DKFZp566O173 antibody
    • FLJ22885 antibody
    • I B22 antibody
    • LYR motif containing protein 3 antibody
    • LYR motif-containing protein 3 antibody
    • LYRM3 antibody
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 antibody
    • NADH ubiquinone oxidoreductase B22 subunit antibody
    • NADH-ubiquinone oxidoreductase B22 subunit antibody
    • NDUB9_HUMAN antibody
    • Ndufb9 antibody
    • UQOR22 antibody
    see all

Images

  • All lanes : Anti-NDUFB9 antibody [EPR15955] (ab188581) at 1/10000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB9 with ab188581 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling NDUFB9 with ab188581 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling NDUFB9 with ab188581 at 1/440 dilution compared to a Rabbit monoclonal IgG isotype control, followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Western blot analysis of NDUFB9 immunoprecipitated from Human fetal liver lysate using ab188581 at 1/50 (Lane 1). Lane 2: PBS instead of Human fetal liver lysates.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

ab188581 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab188581.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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