Anti-NDUFC2 antibody [EPR16499] Recombinant (ab192265)

Product name

Anti-NDUFC2 antibody [EPR16499]
See all NDUFC2 primary antibodies
Description

Rabbit monoclonal [EPR16499] to NDUFC2
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, WB, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant full length protein within Human NDUFC2 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: O95298

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Positive control
- HeLa, HepG2 and A549 cell lysates; Human kidney tissue; A549 cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16499
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab192265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt		1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB		1/1000 - 1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Application

Abreviews

Notes

Flow Cyt

1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB

1/1000 - 1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).

IHC-P

1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Sequence similarities

Belongs to the complex I NDUFC2 subunit family.
Cellular localization

Mitochondrion inner membrane.
Target information above from: UniProt accession O95298 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4718 Human
- Omim: 603845 Human
- SwissProt: O95298 Human
- Unigene: 407860 Human
Alternative names
- B14.5b antibody
- CI-B14.5b antibody
- Complex I B14.5b antibody
- Complex I subunit B14.5b antibody
- Complex I-B14.5b antibody
- HLC-1 antibody
- HLC1 antibody
- Human lung cancer oncogene 1 protein antibody
- NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa antibody
- NADH dehydrogenase [ubiquinone] 1 subunit C2 antibody
- NADH dehydrogenase ubiquinone 1 subunit C2 antibody
- NADH ubiquinone oxidoreductase subunit B14.5b antibody
- NADH-ubiquinone oxidoreductase subunit B14.5b antibody
- NADHDH2 antibody
- NDUC2_HUMAN antibody
- NDUFC2 antibody
see all

Western blot - Anti-NDUFC2 antibody [EPR16499] (ab192265)

All lanes : Anti-NDUFC2 antibody [EPR16499] (ab192265) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A549 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa
Observed band size: 14 kDa

Blocking/Dilution buffer: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFC2 antibody [EPR16499] (ab192265)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFC2 with ab192265 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Flow Cytometry - Anti-NDUFC2 antibody [EPR16499] (ab192265)

Flow cytometric analysis of 2% paraformaldehyde-fixed A549 cells labeling NDUFC2 with ab192265 at 1/30 dilution (pink) compared with a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

ab192265 has not yet been referenced specifically in any publications.

Publishing research using ab192265? Please let us know so that we can cite the reference in this datasheet.

Anti-NDUFC2 antibody [EPR16499] (ab192265)