Recombinant
RabMAb

Recombinant Anti-NDUFC2 antibody [EPR16499] (ab192265)

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Overview

  • Product name

    Anti-NDUFC2 antibody [EPR16499]
    See all NDUFC2 primary antibodies

  • Description

    Rabbit monoclonal [EPR16499] to NDUFC2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein within Human NDUFC2 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: O95298

  • Positive control

    • HeLa, HepG2 and A549 cell lysates; Human kidney tissue; A549 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab192265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
WB 1/1000 - 1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

  • Sequence similarities

    Belongs to the complex I NDUFC2 subunit family.

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • B14.5b antibody
      • CI-B14.5b antibody
      • Complex I B14.5b antibody
      • Complex I subunit B14.5b antibody
      • Complex I-B14.5b antibody
      • HLC-1 antibody
      • HLC1 antibody
      • Human lung cancer oncogene 1 protein antibody
      • NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa antibody
      • NADH dehydrogenase [ubiquinone] 1 subunit C2 antibody
      • NADH dehydrogenase ubiquinone 1 subunit C2 antibody
      • NADH ubiquinone oxidoreductase subunit B14.5b antibody
      • NADH-ubiquinone oxidoreductase subunit B14.5b antibody
      • NADHDH2 antibody
      • NDUC2_HUMAN antibody
      • NDUFC2 antibody
      see all

    Images

    • Western blot - Anti-NDUFC2 antibody [EPR16499] (ab192265)
      Western blot - Anti-NDUFC2 antibody [EPR16499] (ab192265)
      All lanes : Anti-NDUFC2 antibody [EPR16499] (ab192265) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A549 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 14 kDa
      Observed band size: 14 kDa



      Blocking/Dilution buffer: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFC2 antibody [EPR16499] (ab192265)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFC2 antibody [EPR16499] (ab192265)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFC2 with ab192265 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-NDUFC2 antibody [EPR16499] (ab192265)
      Flow Cytometry - Anti-NDUFC2 antibody [EPR16499] (ab192265)

      Flow cytometric analysis of 2% paraformaldehyde-fixed A549 cells labeling NDUFC2 with ab192265 at 1/30 dilution (pink) compared with a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

    References

    ab192265 has not yet been referenced specifically in any publications.

    Publishing research using ab192265? Please let us know so that we can cite the reference in this datasheet.

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