Recombinant Anti-NDUFC2 antibody [EPR16499] (ab192265)
![Western blot - Anti-NDUFC2 antibody [EPR16499] (ab192265)](https://www.abcam.com/ps/products/192/ab192265/Images/ab192265-230107-anti-ndufc2-antibody-epr16499-western-blot.jpg "Western blot - Anti-NDUFC2 antibody [EPR16499] (ab192265)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16499] to NDUFC2
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-NDUFC2 antibody [EPR16499]
See all NDUFC2 primary antibodies -
Description
Rabbit monoclonal [EPR16499] to NDUFC2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, HepG2 and A549 cell lysates; Human kidney tissue; A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16499 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
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| WB |
1/1000 - 1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
Flow Cyt
1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
|
WB
1/1000 - 1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
Sequence similarities
Belongs to the complex I NDUFC2 subunit family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4718 Human
- Omim: 603845 Human
- SwissProt: O95298 Human
- Unigene: 407860 Human
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Alternative names
- B14.5b antibody
- CI-B14.5b antibody
- Complex I B14.5b antibody
see all
Images
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Western blot - Anti-NDUFC2 antibody [EPR16499] (ab192265)All lanes : Anti-NDUFC2 antibody [EPR16499] (ab192265) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFC2 antibody [EPR16499] (ab192265)](https://www.abcam.com/ps/products/192/ab192265/Images/ab192265-230108-anti-ndufc2-antibody-epr16499-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFC2 antibody [EPR16499] (ab192265)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFC2 with ab192265 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-NDUFC2 antibody [EPR16499] (ab192265)](https://www.abcam.com/ps/products/192/ab192265/Images/ab192265-230109-anti-ndufc2-antibody-epr16499-flow-cytometry.jpg)
Flow Cytometry - Anti-NDUFC2 antibody [EPR16499] (ab192265)Flow cytometric analysis of 2% paraformaldehyde-fixed A549 cells labeling NDUFC2 with ab192265 at 1/30 dilution (pink) compared with a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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![Anti-NDUFC2 antibody [EPR16499] (ab192265)](https://www.abcam.com/ps/products/192/ab192265/Images/ab192265-4-benefits-of-recombinant-antibodies.png)
Anti-NDUFC2 antibody [EPR16499] (ab192265)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab192265 has not yet been referenced specifically in any publications.