Product nameAnti-NDUFC2 antibody [EPR16499]
See all NDUFC2 primary antibodies
DescriptionRabbit monoclonal [EPR16499] to NDUFC2
Tested applicationsSuitable for: Flow Cyt, WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein within Human NDUFC2 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: O95298
- HeLa, HepG2 and A549 cell lysates; Human kidney tissue; A549 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab192265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Sequence similaritiesBelongs to the complex I NDUFC2 subunit family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- B14.5b antibody
- CI-B14.5b antibody
- Complex I B14.5b antibody
All lanes : Anti-NDUFC2 antibody [EPR16499] (ab192265) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Blocking/Dilution buffer: 5% NFDM /TBST.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFC2 with ab192265 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow cytometric analysis of 2% paraformaldehyde-fixed A549 cells labeling NDUFC2 with ab192265 at 1/30 dilution (pink) compared with a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
ab192265 has not yet been referenced specifically in any publications.