Product nameAnti-Ndufs1 antibody [EPR11521(B)]
See all Ndufs1 primary antibodies
DescriptionRabbit monoclonal [EPR11521(B)] to Ndufs1
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IP, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human Ndufs1 (P28331).
- Raji, HepG2, HeLa and Jurkat cell lysates. Human cardiac muscle and Human liver tissue. HepG2 and permeabilized Jurkat cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab169540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 79 kDa).|
|IHC-P||1/50 - 1/100.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.
Involvement in diseaseDefects in NDUFS1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
Sequence similaritiesBelongs to the complex I 75 kDa subunit family.
Contains 1 2Fe-2S ferredoxin-type domain.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- CI-75kD antibody
- Complex I 75Kd antibody
- Complex I, mitochondrial respiratory chain, 75 kD subunit antibody
All lanes : Anti-Ndufs1 antibody [EPR11521(B)] (ab169540) at 1/10000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 79 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin embedded Human cardiac muscle tissue labeling Ndufs1 with ab169540 antibody at 1/50.
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling Ndufs1 with ab169540 antibody at 1/50.
Immunofluorescent analysis of HepG2 cells labeling Ndufs1 with ab169540 at 1/100.
Flow Cytometrical analysis of permeabilized Jurkat cells with ab169540 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
Immunoprecipitation. ab169540 at 1/10000 staining Ndufs1 in HeLa cell lysate immunoprecipitated using ab169540 at 1/10. Lane 2: Control IgG. Predicted band size : 79 kDa.
This product has been referenced in:
- Liao X et al. Kruppel-like factor 4 is critical for transcriptional control of cardiac mitochondrial homeostasis. J Clin Invest 125:3461-76 (2015). Read more (PubMed: 26241060) »
- Zeng H et al. High-fat diet induces cardiac remodelling and dysfunction: assessment of the role played by SIRT3 loss. J Cell Mol Med 19:1847-56 (2015). Read more (PubMed: 25782072) »