Overview

  • Product name
    Anti-Ndufs1 antibody [EPR11521(B)]
    See all Ndufs1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11521(B)] to Ndufs1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Ndufs1 (P28331).

  • Positive control
    • Raji, HepG2, HeLa and Jurkat cell lysates. Human cardiac muscle and Human liver tissue. HepG2 and permeabilized Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 79 kDa).
IHC-P 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.
  • Involvement in disease
    Defects in NDUFS1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
  • Sequence similarities
    Belongs to the complex I 75 kDa subunit family.
    Contains 1 2Fe-2S ferredoxin-type domain.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CI-75kD antibody
    • Complex I 75Kd antibody
    • Complex I, mitochondrial respiratory chain, 75 kD subunit antibody
    • Complex I-75kD antibody
    • mitochondrial antibody
    • NADH coenzyme Q reductase antibody
    • NADH dehydrogenase (ubiquinone) FeS protein 1 (75kD) (NADH coenzyme Q reductase) antibody
    • NADH ubiquinone oxidoreductase 75 kDa subunit mitochondrial antibody
    • NADH-ubiquinone oxidoreductase 75 kDa subunit antibody
    • NDUFS1 antibody
    • NDUS1_HUMAN antibody
    • PRO1304 antibody
    see all

Images

  • All lanes : Anti-Ndufs1 antibody [EPR11521(B)] (ab169540) at 1/10000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 79 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin embedded Human cardiac muscle tissue labeling Ndufs1 with ab169540 antibody at 1/50.
  • Immunohistochemical analysis of paraffin embedded Human liver tissue labeling Ndufs1 with ab169540 antibody at 1/50.
  • Immunofluorescent analysis of HepG2 cells labeling Ndufs1 with ab169540 at 1/100.
  • Flow Cytometrical analysis of permeabilized Jurkat cells with ab169540 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
  • Immunoprecipitation. ab169540 at 1/10000 staining Ndufs1 in HeLa cell lysate immunoprecipitated using ab169540 at 1/10. Lane 2: Control IgG. Predicted band size : 79 kDa.

References

This product has been referenced in:
  • Liao X  et al. Kruppel-like factor 4 is critical for transcriptional control of cardiac mitochondrial homeostasis. J Clin Invest 125:3461-76 (2015). Read more (PubMed: 26241060) »
  • Zeng H  et al. High-fat diet induces cardiac remodelling and dysfunction: assessment of the role played by SIRT3 loss. J Cell Mol Med 19:1847-56 (2015). Read more (PubMed: 25782072) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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