RecombinantRabMAb

Recombinant Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

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Western blot - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
  • Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
  • Flow Cytometry - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
  • Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11522(B)] to Ndufs1
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Ndufs1 antibody [EPR11522(B)]
    See all Ndufs1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11522(B)] to Ndufs1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide, corresponding to residues in Human Ndufs1 (UniProt: P28331).

  • Positive control

    • Raji, HepG2, MOLT4 and Jurkat cell lysates; Human kidney and liver tissues; HepG2 cells; Permeabilized Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab157221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 79 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/50 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.

    • Involvement in disease

      Defects in NDUFS1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.

    • Sequence similarities

      Belongs to the complex I 75 kDa subunit family.
      Contains 1 2Fe-2S ferredoxin-type domain.

    • Cellular localization

      Mitochondrion inner membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • CI-75kD antibody
      • Complex I 75Kd antibody
      • Complex I, mitochondrial respiratory chain, 75 kD subunit antibody
      • Complex I-75kD antibody
      • mitochondrial antibody
      • NADH coenzyme Q reductase antibody
      • NADH dehydrogenase (ubiquinone) FeS protein 1 (75kD) (NADH coenzyme Q reductase) antibody
      • NADH ubiquinone oxidoreductase 75 kDa subunit mitochondrial antibody
      • NADH-ubiquinone oxidoreductase 75 kDa subunit antibody
      • NDUFS1 antibody
      • NDUS1_HUMAN antibody
      • PRO1304 antibody
      see all

    Images

    • Western blot - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
      Western blot - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
      All lanes : Anti-Ndufs1 antibody [EPR11522(B)] (ab157221) at 1/10000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : MOLT4 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 79 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
      Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
      Immunofluorescent analysis of HepG2 cells labeling Ndufs1 with ab157221 at 1/50 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

      ab157221 showing +ve staining in Human normal prostate.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

      ab157221 showing +ve staining in Human heart.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

      ab157221 showing +ve staining in Human cervical carcinoma.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

      ab157221 showing +ve staining in Human normal brain.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ndufs1 with ab157221 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ndufs1 with ab157221 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
      Flow Cytometry - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
      Flow cytometric analysis of permeabilized Jurkat cells labeling Ndufs1 with ab157221 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
    • Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)
      Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

    References (2)

    Publishing research using ab157221? Please let us know so that we can cite the reference in this datasheet.

    ab157221 has been referenced in 2 publications.

    • Schweiger M  et al. Pharmacological inhibition of adipose triglyceride lipase corrects high-fat diet-induced insulin resistance and hepatosteatosis in mice. Nat Commun 8:14859 (2017). WB . PubMed: 28327588
    • Petruzzelli M  et al. A switch from white to brown fat increases energy expenditure in cancer-associated cachexia. Cell Metab 20:433-47 (2014). PubMed: 25043816

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