Product nameAnti-Ndufs1 antibody [EPR11522(B)]
See all Ndufs1 primary antibodies
DescriptionRabbit monoclonal [EPR11522(B)] to Ndufs1
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide, corresponding to residues in Human Ndufs1 (UniProt: P28331).
- Raji, HepG2, MOLT4 and Jurkat cell lysates; Human kidney and liver tissues; HepG2 cells; Permeabilized Jurkat cells.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab157221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 79 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/50 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.
Involvement in diseaseDefects in NDUFS1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
Sequence similaritiesBelongs to the complex I 75 kDa subunit family.
Contains 1 2Fe-2S ferredoxin-type domain.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- CI-75kD antibody
- Complex I 75Kd antibody
- Complex I, mitochondrial respiratory chain, 75 kD subunit antibody
All lanes : Anti-Ndufs1 antibody [EPR11522(B)] (ab157221) at 1/10000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : MOLT4 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 79 kDa
Immunofluorescent analysis of HepG2 cells labeling Ndufs1 with ab157221 at 1/50 dilution.
ab157221 showing +ve staining in Human normal prostate.
ab157221 showing +ve staining in Human heart.
ab157221 showing +ve staining in Human cervical carcinoma.
ab157221 showing +ve staining in Human normal brain.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ndufs1 with ab157221 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ndufs1 with ab157221 at 1/100 dilution.
Flow cytometric analysis of permeabilized Jurkat cells labeling Ndufs1 with ab157221 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
This product has been referenced in:
- Schweiger M et al. Pharmacological inhibition of adipose triglyceride lipase corrects high-fat diet-induced insulin resistance and hepatosteatosis in mice. Nat Commun 8:14859 (2017). WB . Read more (PubMed: 28327588) »
- Petruzzelli M et al. A switch from white to brown fat increases energy expenditure in cancer-associated cachexia. Cell Metab 20:433-47 (2014). Read more (PubMed: 25043816) »