Name: Anti-Ndufs1 antibody [EPR11522(B)]
Brand: RabMAb
SKU: ab157221

Product name

Anti-Ndufs1 antibody [EPR11522(B)]
See all Ndufs1 primary antibodies
Description

Rabbit monoclonal [EPR11522(B)] to Ndufs1
Host species

Rabbit
Tested applications

Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide, corresponding to residues in Human Ndufs1 (UniProt: P28331).
Positive control
- Raji, HepG2, MOLT4 and Jurkat cell lysates; Human kidney and liver tissues; HepG2 cells; Permeabilized Jurkat cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11522(B)
Isotype

IgG
Research areas

Alternative Versions
- Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (ab249321)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Recombinant Protein
- Recombinant Human Ndufs1 protein (ab152559)
Related Products
- Recombinant Human Ndufs1 protein (ab152559)

Our Abpromise guarantee covers the use of ab157221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/10000 - 1/50000. Predicted molecular weight: 79 kDa.
Flow Cyt		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF		1/50 - 1/100.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Application notes

Is unsuitable for IP.

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.
Involvement in disease

Defects in NDUFS1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
Sequence similarities

Belongs to the complex I 75 kDa subunit family.
Contains 1 2Fe-2S ferredoxin-type domain.
Cellular localization

Mitochondrion inner membrane.
Target information above from: UniProt accession P28331 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4719 Human
- Entrez Gene: 227197 Mouse
- Entrez Gene: 301458 Rat
- Omim: 157655 Human
- SwissProt: P28331 Human
- SwissProt: Q91VD9 Mouse
- SwissProt: Q66HF1 Rat
- Unigene: 471207 Human
- Unigene: 598436 Human
- Unigene: 290791 Mouse
- Unigene: 1467 Rat
see all
Alternative names
- CI-75kD antibody
- Complex I 75Kd antibody
- Complex I, mitochondrial respiratory chain, 75 kD subunit antibody
- Complex I-75kD antibody
- mitochondrial antibody
- NADH coenzyme Q reductase antibody
- NADH dehydrogenase (ubiquinone) FeS protein 1 (75kD) (NADH coenzyme Q reductase) antibody
- NADH ubiquinone oxidoreductase 75 kDa subunit mitochondrial antibody
- NADH-ubiquinone oxidoreductase 75 kDa subunit antibody
- NDUFS1 antibody
- NDUS1_HUMAN antibody
- PRO1304 antibody
see all

Western blot - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

All lanes : Anti-Ndufs1 antibody [EPR11522(B)] (ab157221) at 1/10000 dilution

Lane 1 : Raji cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : MOLT4 cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 79 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

Immunofluorescent analysis of HepG2 cells labeling Ndufs1 with ab157221 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

ab157221 showing +ve staining in Human normal prostate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

ab157221 showing +ve staining in Human heart.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

ab157221 showing +ve staining in Human cervical carcinoma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

ab157221 showing +ve staining in Human normal brain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ndufs1 with ab157221 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ndufs1 with ab157221 at 1/100 dilution.
Flow Cytometry - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

Flow cytometric analysis of permeabilized Jurkat cells labeling Ndufs1 with ab157221 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Schweiger M et al. Pharmacological inhibition of adipose triglyceride lipase corrects high-fat diet-induced insulin resistance and hepatosteatosis in mice. Nat Commun 8:14859 (2017). WB . Read more (PubMed: 28327588) »
Petruzzelli M et al. A switch from white to brown fat increases energy expenditure in cancer-associated cachexia. Cell Metab 20:433-47 (2014). Read more (PubMed: 25043816) »

See all 2 Publications for this product

Publishing research using ab157221? Please let us know so that we can cite the reference in this datasheet.

Location selector

Your location is currently set to:

Custom solutions & partnerships

Recombinant Anti-Ndufs1 antibody [EPR11522(B)] (ab157221)

Overview

Properties

Associated products

Applications

Target

Images

Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As