Product nameAnti-NDUFS2 antibody [7A12BE5AD5]
See all NDUFS2 primary antibodies
DescriptionMouse monoclonal [7A12BE5AD5] to NDUFS2
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Tissue, cells or virus. This information is considered to be commercially sensitive.
- WB: Isolated mitochondria from Human liver, Bovine heart, H9C2 cells (Rat cardiomyocyte) and MEF cells (Mouse embryo fibroblast) ICC/IF: HDFn cultured cells (normal Human dermal fibroblasts, neonatal)
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
Purification notesThe purity of ab110249 is near homogeneity, as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab110249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.25 µg/ml. Predicted molecular weight: 53 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Involvement in diseaseDefects in NDUFS2 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
Sequence similaritiesBelongs to the complex I 49 kDa subunit family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- CI 49 antibody
- CI 49kD antibody
- CI-49kD antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: NDUFS2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab110249 observed at 48 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab110249 was shown to specifically react with NDUFS2 in wild-type HAP1 cells whilst signal was lost in NDUFS2 knockout cells. Wild-type and NDUFS2 knockout samples were subjected to SDS-PAGE. ab110249 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 0.25 µg/ml and 1/20,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
Overlay histogram showing HepG2 cells stained with ab110249 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110249, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
All lanes : Anti-NDUFS2 antibody [7A12BE5AD5] (ab110249) at 0.25 µg/ml
Lane 1 : Molecular Weight Markers
Lane 2 : Isolated mitochondria from Human liver
Lane 3 : Isolated mitochondria from Bovine heart
Lane 4 : Isolated mitochondria from H9C2 cells (Rat cardiomyocyte)
Lane 5 : Isolated mitochondria from MEF cells (Mouse embryo fibroblast)
Lane 6 : Isolated mitochondria from HepG2
Predicted band size: 53 kDa
Complex I-subunit NDUFS2 is detected in the Human samples with Human specific ab110249, while Complex I-subunit NDUFB8 is detected in all samples (Human, Mouse and Rat) with an anti-NDUFB8.
Mitochondrial localization of Complex I subunit NDUFS2 visualized by Immunocytochemistry in HDFn cultured cells (normal Human dermal fibroblasts, neonatal), using ab110249 at 5 µg/ml.
This product has been referenced in:
- Hernansanz-Agustín P et al. Mitochondrial complex I deactivation is related to superoxide production in acute hypoxia. Redox Biol 12:1040-1051 (2017). WB . Read more (PubMed: 28511347) »
- Wakim J et al. CLUH couples mitochondrial distribution to the energetic and metabolic status. J Cell Sci 130:1940-1951 (2017). Read more (PubMed: 28424233) »