Anti-NDUFS2 antibody (ab192764)
Key features and details
- Rabbit polyclonal to NDUFS2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NDUFS2 antibody
See all NDUFS2 primary antibodies -
Description
Rabbit polyclonal to NDUFS2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human NDUFS2 aa 200-450. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O75306 -
Positive control
- Human kidney tissue; HeLa cells; 293T, A431, Jurkat, Raji cell lysate, Mouse brain tissue lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 79% Tris glycine -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
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IHC-P |
Use a concentration of 7.5 µg/ml.
Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0) |
Notes |
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ICC/IF
1/100 - 1/1000. |
WB
1/1000 - 1/10000. Predicted molecular weight: 53 kDa. |
IHC-P
Use a concentration of 7.5 µg/ml. Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0) |
Target
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Function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
Involvement in disease
Defects in NDUFS2 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
Sequence similarities
Belongs to the complex I 49 kDa subunit family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4720 Human
- Entrez Gene: 226646 Mouse
- Omim: 602985 Human
- SwissProt: O75306 Human
- SwissProt: Q91WD5 Mouse
- Unigene: 173611 Human
- Unigene: 21669 Mouse
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Alternative names
- CI 49 antibody
- CI 49kD antibody
- CI-49kD antibody
see all
Images
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All lanes : Anti-NDUFS2 antibody (ab192764) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa -
Anti-NDUFS2 antibody (ab192764) at 1/10000 dilution + Mouse brain tissue lysate at 50 µg
Predicted band size: 53 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling NDUFS2 with ab192764 at 7.5μg/mL.
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Immunofluorescent analysis of HeLa cells labeling mitochondrial NDUFS2 with ab192764 at a 1/500 dilution. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37 for 30 min. The panel on the right shows the nuclear stain.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab192764 has not yet been referenced specifically in any publications.