Key features and details
- Rabbit polyclonal to NDUFS2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NDUFS2 antibody
See all NDUFS2 primary antibodies
DescriptionRabbit polyclonal to NDUFS2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human NDUFS2 aa 200-430. The exact sequence is proprietary.
F FWLFEEREKM FEFYERVSGA RMHAAYIRPG GVHQDLPLGL MDDIYQFSKN FSLRLDELEE LLTNNRIWRN RTIDIGVVTA EEALNYGFSG VMLRGSGIQW DLRKTQPYDV YDQVEFDVPV GSRGDCYDRY LCRVEEMRQS LRIIAQCLNK MPPGEIKVDD AKVSPPKRAE MKTSMESLIH HFKLYTEGYQ VPPGATYTAI EAPKGEFGVY LVSDGSSRPY RCKIKAPGFA
Database link: O75306
- Human kidney tissue; HeLa cells; 293T, A431, Jurkat, Raji cell lysate, Mouse brain tissue lysate
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 79% Tris glycine
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab192764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 53 kDa.|
|IHC-P||Use a concentration of 7.5 µg/ml.
Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)
FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Involvement in diseaseDefects in NDUFS2 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
Sequence similaritiesBelongs to the complex I 49 kDa subunit family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- CI 49 antibody
- CI 49kD antibody
- CI-49kD antibody
All lanes : Anti-NDUFS2 antibody (ab192764) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
Anti-NDUFS2 antibody (ab192764) at 1/10000 dilution + Mouse brain tissue lysate at 50 µg
Predicted band size: 53 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling NDUFS2 with ab192764 at 7.5μg/mL.
Immunofluorescent analysis of HeLa cells labeling mitochondrial NDUFS2 with ab192764 at a 1/500 dilution. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37 for 30 min. The panel on the right shows the nuclear stain.
ab192764 has not yet been referenced specifically in any publications.