Anti-NDUFS2 antibody [EPR16266] (ab192022)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-NDUFS2 antibody [EPR16266]
See all NDUFS2 primary antibodies -
DescriptionRabbit monoclonal [EPR16266] to NDUFS2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, IPmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment within Human NDUFS2 aa 300-450. The exact sequence is proprietary.
Database link: O75306 -
Positive control
- HeLa, Jurkat, 293T cell lysate. Mouse brain, kidney and spleen lysate. Rat brain and kidney lysate. C6, RAW264.7, PC-12 and NIH/3T3 cell lysate. Human brain tissue. Rat stomach tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR16266
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- Rat brain normal tissue lysate - total protein (ab29475)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- Mouse brain tissue lysate - total protein (ab30151)
- NIH 3T3 whole cell lysate (ab7179)
- RAW 264.7 whole cell lysate (ab7187)
- Jurkat whole cell lysate (ab7899)
- Mouse spleen tissue lysate - total protein (ab7937)
- 293T whole cell lysate (ab95494)
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Related Products
Applications
Our Abpromise guarantee covers the use of ab192022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa). | |
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| IP | 1/40. |
Target
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FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
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Involvement in diseaseDefects in NDUFS2 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
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Sequence similaritiesBelongs to the complex I 49 kDa subunit family.
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Cellular localizationMitochondrion inner membrane.
- Information by UniProt
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Database links
- Entrez Gene: 4720 Human
- Entrez Gene: 226646 Mouse
- Entrez Gene: 289218 Rat
- Omim: 602985 Human
- SwissProt: O75306 Human
- SwissProt: Q91WD5 Mouse
- SwissProt: Q641Y2 Rat
- Unigene: 173611 Human
see all -
Alternative names
- CI 49 antibody
- CI 49kD antibody
- CI-49kD antibody
see all
Images
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![Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)](https://www.abcam.com/ps/products/192/ab192022/Images/ab192022-285152-ab192022GDBKOWB.jpg)
Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: NDUFS2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab192022 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab192022 was shown to specifically react with NDUFS2 in wild-type HAP1 cells whilst signal was lost in NDUFS2 knockout cells. Wild-type and NDUFS2 knockout samples were subjected to SDS-PAGE. ab192022 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (ab192022)](https://www.abcam.com/ps/products/192/ab192022/Images/ab192022-230793-ab1920223.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (ab192022)Immunohistochemical analysis of paraffin-embedded human brain tissue sections labeling NDUFS2 using ab192022 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.
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![Immunoprecipitation - Anti-NDUFS2 antibody [EPR16266] (ab192022)](https://www.abcam.com/ps/products/192/ab192022/Images/ab192022-230791-ab1920225.jpg)
Immunoprecipitation - Anti-NDUFS2 antibody [EPR16266] (ab192022)Lane 1: 293T cell lysate was immunoprecipitated using ab192022 at a 1/40 dilution. Secondary used was anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution.
Lane 2: PBS instead of 293T lysates.
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![Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)](https://www.abcam.com/ps/products/192/ab192022/Images/ab192022-230797-ab1920221.jpg)
Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)All lanes : Anti-NDUFS2 antibody [EPR16266] (ab192022) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (ab192022)](https://www.abcam.com/ps/products/192/ab192022/Images/ab192022-230792-ab1920224.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (ab192022)Immunohistochemical analysis of paraffin-embedded rat stomach tissue sections labeling NDUFS2 using ab192022 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.
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![Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)](https://www.abcam.com/ps/products/192/ab192022/Images/ab192022-230796-ab1920222.jpg)
Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)All lanes : Anti-NDUFS2 antibody [EPR16266] (ab192022) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat kidney lysate
Lane 6 : C6 cell lysate
Lane 7 : RAW264.7 cell lysate
Lane 8 : PC-12 cell lysate
Lane 9 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 49 kDa (why is the actual band size different from the predicted?)
References
ab192022 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"