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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-NDUFS2 antibody [EPR16266] (ab192022)

Submit a review Submit a question References (3)
Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (ab192022)
  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFS2 antibody [EPR16266] (ab192022)
  • Immunoprecipitation - Anti-NDUFS2 antibody [EPR16266] (ab192022)
  • Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (ab192022)
  • Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)
  • Anti-NDUFS2 antibody [EPR16266] (ab192022)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16266] to NDUFS2
  • Suitable for: ICC/IF, WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-NDUFS2 antibody [EPR16266]
    See all NDUFS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR16266] to NDUFS2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HAP1, HeLa, Jurkat, 293T cell lysate. Mouse brain, kidney and spleen lysate. Rat brain and kidney lysate. C6, RAW264.7, PC-12 and NIH/3T3 cell lysate. Human brain tissue. Rat stomach tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab192022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/1000.
WB
1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa).
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/40.
Notes
ICC/IF
1/1000.
WB
1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa).
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/40.

Target

  • Function

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

  • Involvement in disease

    Defects in NDUFS2 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.

  • Sequence similarities

    Belongs to the complex I 49 kDa subunit family.

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CI 49 antibody
    • CI 49kD antibody
    • CI-49kD antibody
    • Complex 1, mitochondrial respiratory chain, 49 KD subunit antibody
    • Complex I 49kD antibody
    • Complex I 49kDa subunit antibody
    • Complex I-49kD antibody
    • mitochondrial antibody
    • NADH dehydrogenase (ubiquinone) Fe S protein 2 49kDa antibody
    • NADH dehydrogenase (ubiquinone) Fe S protein 2, 49kDa (NADH coenzyme Q reductase) antibody
    • NADH dehydrogenase [ubiquinone] iron sulfur protein 2, mitochondrial antibody
    • NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 antibody
    • NADH ubiquinone oxidoreductase 49 kDa subunit antibody
    • NADH ubiquinone oxidoreductase NDUFS2 subunit antibody
    • NADH-ubiquinone oxidoreductase 49 kDa subunit antibody
    • NADH:ubiquinone oxidoreductase core subunit S2 antibody
    • Ndufs2 antibody
    • NDUS2_HUMAN antibody
    see all

Images

  • Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: NDUFS2 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab192022 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab192022 was shown to specifically react with NDUFS2 in wild-type HAP1 cells whilst signal was lost in NDUFS2 knockout cells. Wild-type and NDUFS2 knockout samples were subjected to SDS-PAGE. ab192022 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (ab192022)

    Immunohistochemical analysis of paraffin-embedded human brain tissue sections labeling NDUFS2 using ab192022 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    Immunocytochemistry/ Immunofluorescence - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    ab192022 staining NDUFS2 in wild-type Hap1 cells (top panel) and NDUFS2 knockout Hap1 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192022 at 0.4μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
  • Immunoprecipitation - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    Immunoprecipitation - Anti-NDUFS2 antibody [EPR16266] (ab192022)

    Lane 1: 293T cell lysate was immunoprecipitated using ab192022 at a 1/40 dilution. Secondary used was anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution.

    Lane 2: PBS instead of 293T lysates.

  • Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    All lanes : Anti-NDUFS2 antibody [EPR16266] (ab192022) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (ab192022)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue sections labeling NDUFS2 using ab192022 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    All lanes : Anti-NDUFS2 antibody [EPR16266] (ab192022) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat kidney lysate
    Lane 6 : C6 cell lysate
    Lane 7 : RAW264.7 cell lysate
    Lane 8 : PC-12 cell lysate
    Lane 9 : NIH3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 49 kDa why is the actual band size different from the predicted?

  • Anti-NDUFS2 antibody [EPR16266] (ab192022)
    Anti-NDUFS2 antibody [EPR16266] (ab192022)

References (3)

Publishing research using ab192022? Please let us know so that we can cite the reference in this datasheet.

ab192022 has been referenced in 3 publications.

  • Ruan QT  et al. A Mutation in Hnrnph1 That Decreases Methamphetamine-Induced Reinforcement, Reward, and Dopamine Release and Increases Synaptosomal hnRNP H and Mitochondrial Proteins. J Neurosci 40:107-130 (2020). PubMed: 31704785
  • Jing YY  et al. Epigenetic regulation of the Warburg effect by H2B monoubiquitination. Cell Death Differ 27:1660-1676 (2020). PubMed: 31685978
  • Gregg T  et al. Obesity-dependent CDK1 signaling stimulates mitochondrial respiration at complex I in pancreatic ß-cells. J Biol Chem 294:4656-4666 (2019). PubMed: 30700550

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