Anti-NDUFS3 antibody [3F9DD2] (ab110246)
- Datasheet
- References (46)
- Protocols
Overview
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Product nameAnti-NDUFS3 antibody [3F9DD2]
See all NDUFS3 primary antibodies -
DescriptionMouse monoclonal [3F9DD2] to NDUFS3
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Host speciesMouse
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Tested applicationsSuitable for: IHC-Fr, IHC-Glut, Flow Cyt, ICC/IF, IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Rat, Cow, Human, Zebrafish
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Immunogen
Whole mitochondria from Human Heart.
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Positive control
- Isolated mitochondria from Human, Bovine Rat and Mouse hearts; Cultured Human fibroblasts; HeLa cells; Human colon and cerebellum tissues.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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PurityIgG fraction
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Purification notesab110246 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110246 is judged as near homogeneity by SDS PAGE.
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ClonalityMonoclonal
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Clone number3F9DD2
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab110246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr | Use at an assay dependent concentration. PubMed: 25223649 | |
IHC-Glut | 1/100. | |
Flow Cyt | Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF | Use a concentration of 5 - 10 µg/ml. | |
IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. | |
WB | Use a concentration of 0.5 µg/ml. Predicted molecular weight: 30 kDa. |
Target
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FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
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Sequence similaritiesBelongs to the complex I 30 kDa subunit family.
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Cellular localizationMitochondrion inner membrane.
- Information by UniProt
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Database links
- Entrez Gene: 287327 Cow
- Entrez Gene: 4722 Human
- Entrez Gene: 68349 Mouse
- Entrez Gene: 295923 Rat
- Entrez Gene: 550451 Zebrafish
- Omim: 603846 Human
- SwissProt: P23709 Cow
- SwissProt: O75489 Human
see all -
Alternative names
- CI 30 antibody
- CI 30KD antibody
- CI-30kD antibody
see all
Images
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Western blot - Anti-NDUFS3 antibody [3F9DD2] (ab110246)This image is courtesy of an anonymous AbReviewAll lanes : Anti-NDUFS3 antibody [3F9DD2] (ab110246) at 1/1000 dilution
Lanes 1-2 : Whole cell lysates from NDUFS3 KO cells.
Lanes 3-4 : Whole cell lysates from NDUFS3 WT cells.
Lanes 5-6 : Isolated mitochondria from NDUFS3 KO cells.
Lanes 7-8 : Isolated mitochondria from NDUFS3 WT cells.
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Mouse monoclonal HRP conjugate at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Additional bands at: 260 kDa (possible non-specific binding), 40 kDa (possible non-specific binding)
Exposure time: 1 minute
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All lanes : Anti-NDUFS3 antibody [3F9DD2] (ab110246) at 0.5 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
Predicted band size: 30 kDa -
ab110246, at 10 µg/ml, staining NDUFS3 in cultured Human fibroblasts by Immunofluorescence. The cells were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110246, followed by a fluorescent goat-anti-mouse IgG.
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ab110246, at 1 µg/ml, staining NDUFS3 in HeLa cells by Flow Cytometry (Blue).
Isotype control antibody, at 1 µg/ml, staining (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)ab110246, at 1/100, staining NDUFS3 in Human colon tissue fixed with 4% PFA by Immunohistochemistry.
ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated for 1 hour with the target. Antigen retrieval was not required.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)ab110246, at 1/1000, staining NDUFS3 in formalin fixed and paraffin embedded Human cerebellum tissue by Immunohistochemistry.
ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated with the target for 1 hour. Antigen retrieval performed by 1 min pressure cooking with 1mmol EDTA, pH8.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)ab110246 staining NDUFS3 in Human colon tissue sections from a normal ageing patient by Immunohistochemistry. Kindly provided by Dr. L. Greaves and D. Turnbull, Mitochondrial Research Group, Newcastle University. For more details, see Taylor et al., J. Clin. Invest. 112:1351-1360 (2003).
Protocols
References
This product has been referenced in:
- Lee YL et al. Epigallocatechin-3-gallate alleviates bladder overactivity in a rat model with metabolic syndrome and ovarian hormone deficiency through mitochondria apoptosis pathways. Sci Rep 8:5358 (2018). Read more (PubMed: 29599473) »
- Franco-Iborra S et al. Defective mitochondrial protein import contributes to complex I-induced mitochondrial dysfunction and neurodegeneration in Parkinson's disease. Cell Death Dis 9:1122 (2018). Read more (PubMed: 30405116) »