Western blot abreview for Anti-NDUFS3 antibody [EPR12782] - C-terminal

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (osteosarcoma)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
osteosarcoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS 1X Tween 0.05% milk 5%
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: ANTI RABBIT
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Negative control
NDUFS3 KO cells
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 30 kDa
Positive control
NDUFS3 WT cells

Additional data

Additional Notes
On nitrocellulose membrane the antibody recognizes the specific NDUFS3 band ONLY (verified by knocking out NDUFS3 by genome editing). Samples prepared with proteases and phosphatases inhibitors. Performed on whole cell lysates and isolated mitochondria. Sample loading: 1. whole cell lysates from NDUFS3 KO cells 2. whole cell lysates from NDUFS3 KO cells 3. whole cell lysates from NDUFS3 WT cells 4. whole cell lysates from NDUFS3 WT cells 5. isolated mitochondria from NDUFS3 KO cells 6. isolated mitochondria from NDUFS3 KO cells 7. isolated mitochondria from NDUFS3 WT cells 8. isolated mitochondria from NDUFS3 WT cells

Dr. Luisa Iommarini

Verified customer

Submitted Nov 03 2015

Sign up