Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ndufs4 antibody [EP7832]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/4000

Secondary antibody

Dilution
1/300
Name
Non-Abcam antibody was used: anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Submitted image of kidney cortex shows intense basal cytoplasmic positivity in proximal convoluted tubules - lumenal cytoplasm is negative. This pattern conforms to known data (also, the same pattern is seen in human kidney using ab14705). Distal convoluted tubules show a less intense positivity, mostly throughout the cytoplasm.

Mr. Carl Hobbs

Verified customer

Submitted Feb 11 2019

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