Application
Western blot
Sample
Human Cell lysate - whole cell (HEK-293)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (8-12% Bis-Tris)
Loading amount
40 µg
Specification
HEK-293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 4%
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Mouse anti rabbit
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
SuperSignal™ West Femto Maximum Sensitiv
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 20 kDa
Abcam user community
Verified customer
Submitted Jan 25 2021