Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Ndufs4 antibody [EPR7831] (ab139178)

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Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
  • Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
  • Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)
  • Anti-Ndufs4 antibody [EPR7831] (ab139178)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7831] to Ndufs4
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ndufs4 antibody [EPR7831]
    See all Ndufs4 primary antibodies

  • Description

    Rabbit monoclonal [EPR7831] to Ndufs4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Ndufs4 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human fetal heart, Human fetal kidney, Human fetal brain, 293T, Mouse heart, Mouse kidney and Rat kidney lysates; Human kidney and stomach tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab139178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Mouse
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized antibody recommended.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized antibody recommended.
  • Application notes
    Is unsuitable for ICC.

    • Target

    • Function

      Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

    • Involvement in disease

      Defects in NDUFS4 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.

    • Sequence similarities

      Belongs to the complex I NDUFS4 subunit family.

    • Cellular localization

      Mitochondrion inner membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • AQDQ antibody
      • CI 18 antibody
      • CI 18 kDa antibody
      • CI AQDQ antibody
      • CI-18 kDa antibody
      • CI-AQDQ antibody
      • Complex I 18 kDa antibody
      • Complex I AQDQ antibody
      • Complex I-18 kDa antibody
      • Complex I-AQDQ antibody
      • mitochondrial antibody
      • mitochondrial respiratory chain complex I (18 KD subunit) antibody
      • NADH coenzyme Q reductase antibody
      • NADH dehydrogenase (ubiquinone) Fe S protein 4 18kDa antibody
      • NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 antibody
      • NADH dehydrogenase antibody
      • NADH ubiquinone oxidoreductase 18 kDa subunit antibody
      • NADH-ubiquinone oxidoreductase 18 kDa subunit antibody
      • NDUFS4 antibody
      • NDUS4_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Ndufs4knockout HAP1 cell lysate (20 µg)
      Lane 3: HEK293 cell lysate (20 µg)
      Lane 4: Hu fetal heart tissue lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab139178 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab139178 was shown to recognize Ndufs4 when Ndufs4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Ndufs4 knockout samples were subjected to SDS-PAGE. ab139178 and ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      All lanes : Anti-Ndufs4 antibody [EPR7831] (ab139178) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : Human fetal brain lysate
      Lane 4 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit

      Predicted band size: 20 kDa

    • Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      All lanes : Anti-Ndufs4 antibody [EPR7831] (ab139178) at 1/1000 dilution

      Lane 1 : Mouse heart lysate
      Lane 2 : Mouse kidney lysate
      Lane 3 : Rat kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 20 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Ndufs4 with ab139178 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)

      Immunohistochemical analysis of paraffin embedded Human stomach tissue labelling Ndufs4 with ab139178 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Anti-Ndufs4 antibody [EPR7831] (ab139178)

    References (4)

    Publishing research using ab139178? Please let us know so that we can cite the reference in this datasheet.

    ab139178 has been referenced in 4 publications.

    • Sanchez-Ruderisch H  et al. Sex-specific regulation of cardiac microRNAs targeting mitochondrial proteins in pressure overload. Biol Sex Differ 10:8 (2019). PubMed: 30728084
    • Zhao X  et al. JS-K induces reactive oxygen species-dependent anti-cancer effects by targeting mitochondria respiratory chain complexes in gastric cancer. J Cell Mol Med 23:2489-2504 (2019). PubMed: 30672108
    • Tani H  et al. Disruption of the mouse Shmt2 gene confers embryonic anaemia via foetal liver-specific metabolomic disorders. Sci Rep 9:16054 (2019). PubMed: 31690790
    • Morscher RJ  et al. Mitochondrial translation requires folate-dependent tRNA methylation. Nature 554:128-132 (2018). PubMed: 29364879

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