Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Ndufs4 antibody [EPR7831] (ab139178)

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Overview

  • Product name

    Anti-Ndufs4 antibody [EPR7831]
    See all Ndufs4 primary antibodies

  • Description

    Rabbit monoclonal [EPR7831] to Ndufs4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IP, Flow Cytmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Ndufs4 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human fetal heart, Human fetal kidney, Human fetal brain, 293T, Mouse heart, Mouse kidney and Rat kidney lysates; Human kidney and stomach tissues.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab139178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized antibody recommended.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC.

    • Target

    • Function

      Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

    • Involvement in disease

      Defects in NDUFS4 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.

    • Sequence similarities

      Belongs to the complex I NDUFS4 subunit family.

    • Cellular localization

      Mitochondrion inner membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • AQDQ antibody
      • CI 18 antibody
      • CI 18 kDa antibody
      • CI AQDQ antibody
      • CI-18 kDa antibody
      • CI-AQDQ antibody
      • Complex I 18 kDa antibody
      • Complex I AQDQ antibody
      • Complex I-18 kDa antibody
      • Complex I-AQDQ antibody
      • mitochondrial antibody
      • mitochondrial respiratory chain complex I (18 KD subunit) antibody
      • NADH coenzyme Q reductase antibody
      • NADH dehydrogenase (ubiquinone) Fe S protein 4 18kDa antibody
      • NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 antibody
      • NADH dehydrogenase antibody
      • NADH ubiquinone oxidoreductase 18 kDa subunit antibody
      • NADH-ubiquinone oxidoreductase 18 kDa subunit antibody
      • NDUFS4 antibody
      • NDUS4_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Ndufs4knockout HAP1 cell lysate (20 µg)
      Lane 3: HEK293 cell lysate (20 µg)
      Lane 4: Hu fetal heart tissue lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab139178 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab139178 was shown to recognize Ndufs4 when Ndufs4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Ndufs4 knockout samples were subjected to SDS-PAGE. ab139178 and ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      All lanes : Anti-Ndufs4 antibody [EPR7831] (ab139178) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : Human fetal brain lysate
      Lane 4 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit

      Predicted band size: 20 kDa

    • Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      All lanes : Anti-Ndufs4 antibody [EPR7831] (ab139178) at 1/1000 dilution

      Lane 1 : Mouse heart lysate
      Lane 2 : Mouse kidney lysate
      Lane 3 : Rat kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 20 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Ndufs4 with ab139178 at 1/50 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Immunohistochemical analysis of paraffin embedded Human stomach tissue labelling Ndufs4 with ab139178 at 1/50 dilution.

    References

    This product has been referenced in:

    • Sanchez-Ruderisch H  et al. Sex-specific regulation of cardiac microRNAs targeting mitochondrial proteins in pressure overload. Biol Sex Differ 10:8 (2019). Read more (PubMed: 30728084) »
    • Zhao X  et al. JS-K induces reactive oxygen species-dependent anti-cancer effects by targeting mitochondria respiratory chain complexes in gastric cancer. J Cell Mol Med 23:2489-2504 (2019). Read more (PubMed: 30672108) »
    See all 3 Publications for this product

    Publishing research using ab139178? Please let us know so that we can cite the reference in this datasheet.

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