Recombinant Anti-Ndufs4 antibody [EPR7831] (ab139178)
![Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)](https://www.abcam.com/ps/products/139/ab139178/Images/ab139178-260995-anti-ndufs4-antibody-epr7831-western-blot.jpg "Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7831] to Ndufs4
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Ndufs4 antibody [EPR7831]
See all Ndufs4 primary antibodies -
Description
Rabbit monoclonal [EPR7831] to Ndufs4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Ndufs4 aa 50-150. The exact sequence is proprietary.
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Positive control
- Human fetal heart, Human fetal kidney, Human fetal brain, 293T, Mouse heart, Mouse kidney and Rat kidney lysates; Human kidney and stomach tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7831 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized antibody recommended.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized antibody recommended. |
Target
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Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
Involvement in disease
Defects in NDUFS4 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
Sequence similarities
Belongs to the complex I NDUFS4 subunit family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4724 Human
- Entrez Gene: 17993 Mouse
- Entrez Gene: 499529 Rat
- Omim: 602694 Human
- SwissProt: O43181 Human
- SwissProt: Q9CXZ1 Mouse
- SwissProt: Q5XIF3 Rat
- Unigene: 528222 Human
see all -
Alternative names
- AQDQ antibody
- CI 18 antibody
- CI 18 kDa antibody
see all
Images
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Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Ndufs4knockout HAP1 cell lysate (20 µg)
Lane 3: HEK293 cell lysate (20 µg)
Lane 4: Hu fetal heart tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab139178 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab139178 was shown to recognize Ndufs4 when Ndufs4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Ndufs4 knockout samples were subjected to SDS-PAGE. ab139178 and ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)](https://www.abcam.com/ps/products/139/ab139178/Images/ab139178-154387-anti-ndufs4-antibody-epr7831-western-blot.jpg)
Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)All lanes : Anti-Ndufs4 antibody [EPR7831] (ab139178) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal brain lysate
Lane 4 : 293T lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit
Predicted band size: 20 kDa -
![Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)](https://www.abcam.com/ps/products/139/ab139178/Images/ab139178-154386-anti-ndufs4-antibody-epr7831-western-blot.jpg)
Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)All lanes : Anti-Ndufs4 antibody [EPR7831] (ab139178) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 20 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)](https://www.abcam.com/ps/products/139/ab139178/Images/ab139178-154385-anti-ndufs4-antibody-epr7831-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Ndufs4 with ab139178 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)](https://www.abcam.com/ps/products/139/ab139178/Images/ab139178-154384-anti-ndufs4-antibody-epr7831-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)Immunohistochemical analysis of paraffin embedded Human stomach tissue labelling Ndufs4 with ab139178 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Ndufs4 antibody [EPR7831] (ab139178)](https://www.abcam.com/ps/products/139/ab139178/Images/ab139178-6-benefits-of-recombinant-antibodies.png)
Anti-Ndufs4 antibody [EPR7831] (ab139178)
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab139178 has been referenced in 4 publications.
- Sanchez-Ruderisch H et al. Sex-specific regulation of cardiac microRNAs targeting mitochondrial proteins in pressure overload. Biol Sex Differ 10:8 (2019). PubMed: 30728084
- Zhao X et al. JS-K induces reactive oxygen species-dependent anti-cancer effects by targeting mitochondria respiratory chain complexes in gastric cancer. J Cell Mol Med 23:2489-2504 (2019). PubMed: 30672108
- Tani H et al. Disruption of the mouse Shmt2 gene confers embryonic anaemia via foetal liver-specific metabolomic disorders. Sci Rep 9:16054 (2019). PubMed: 31690790
- Morscher RJ et al. Mitochondrial translation requires folate-dependent tRNA methylation. Nature 554:128-132 (2018). PubMed: 29364879
