For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    ndufs4-antibody-epr7831-ab139178.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Metabolism Energy Metabolism
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Ndufs4 antibody [EPR7831] (ab139178)

  • Datasheet
  • SDS
Submit a review Submit a question References (4)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
  • Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
  • Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)
  • Anti-Ndufs4 antibody [EPR7831] (ab139178)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7831] to Ndufs4
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
Product image
Recombinant Human Ndufs4 protein (ab86684)

View more associated products

Overview

  • Product name

    Anti-Ndufs4 antibody [EPR7831]
    See all Ndufs4 primary antibodies
  • Description

    Rabbit monoclonal [EPR7831] to Ndufs4
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Ndufs4 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human fetal heart, Human fetal kidney, Human fetal brain, 293T, Mouse heart, Mouse kidney and Rat kidney lysates; Human kidney and stomach tissues.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7831
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex I

Associated products

  • Alternative Versions

    • Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (ab248861)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse kidney normal tissue lysate - total protein (ab29305)
  • Recombinant Protein

    • Recombinant Human Ndufs4 protein (ab86684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab139178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Mouse
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized antibody recommended.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized antibody recommended.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function

      Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
    • Involvement in disease

      Defects in NDUFS4 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
    • Sequence similarities

      Belongs to the complex I NDUFS4 subunit family.
    • Cellular localization

      Mitochondrion inner membrane.
    • Target information above from: UniProt accession O43181 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4724 Human
      • Entrez Gene: 17993 Mouse
      • Entrez Gene: 499529 Rat
      • Omim: 602694 Human
      • SwissProt: O43181 Human
      • SwissProt: Q9CXZ1 Mouse
      • SwissProt: Q5XIF3 Rat
      • Unigene: 528222 Human
      • Unigene: 253142 Mouse
      • Unigene: 203141 Rat
      see all
    • Alternative names

      • AQDQ antibody
      • CI 18 antibody
      • CI 18 kDa antibody
      • CI AQDQ antibody
      • CI-18 kDa antibody
      • CI-AQDQ antibody
      • Complex I 18 kDa antibody
      • Complex I AQDQ antibody
      • Complex I-18 kDa antibody
      • Complex I-AQDQ antibody
      • mitochondrial antibody
      • mitochondrial respiratory chain complex I (18 KD subunit) antibody
      • NADH coenzyme Q reductase antibody
      • NADH dehydrogenase (ubiquinone) Fe S protein 4 18kDa antibody
      • NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 antibody
      • NADH dehydrogenase antibody
      • NADH ubiquinone oxidoreductase 18 kDa subunit antibody
      • NADH-ubiquinone oxidoreductase 18 kDa subunit antibody
      • NDUFS4 antibody
      • NDUS4_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Ndufs4knockout HAP1 cell lysate (20 µg)
      Lane 3: HEK293 cell lysate (20 µg)
      Lane 4: Hu fetal heart tissue lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab139178 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab139178 was shown to recognize Ndufs4 when Ndufs4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Ndufs4 knockout samples were subjected to SDS-PAGE. ab139178 and ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      All lanes : Anti-Ndufs4 antibody [EPR7831] (ab139178) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : Human fetal brain lysate
      Lane 4 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit

      Predicted band size: 20 kDa

    • Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Western blot - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      All lanes : Anti-Ndufs4 antibody [EPR7831] (ab139178) at 1/1000 dilution

      Lane 1 : Mouse heart lysate
      Lane 2 : Mouse kidney lysate
      Lane 3 : Rat kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 20 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Ndufs4 with ab139178 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] (ab139178)

      Immunohistochemical analysis of paraffin embedded Human stomach tissue labelling Ndufs4 with ab139178 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Ndufs4 antibody [EPR7831] (ab139178)
      Anti-Ndufs4 antibody [EPR7831] (ab139178)

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab139178? Please let us know so that we can cite the reference in this datasheet.

    ab139178 has been referenced in 4 publications.

    • Sanchez-Ruderisch H  et al. Sex-specific regulation of cardiac microRNAs targeting mitochondrial proteins in pressure overload. Biol Sex Differ 10:8 (2019). PubMed: 30728084
    • Zhao X  et al. JS-K induces reactive oxygen species-dependent anti-cancer effects by targeting mitochondria respiratory chain complexes in gastric cancer. J Cell Mol Med 23:2489-2504 (2019). PubMed: 30672108
    • Tani H  et al. Disruption of the mouse Shmt2 gene confers embryonic anaemia via foetal liver-specific metabolomic disorders. Sci Rep 9:16054 (2019). PubMed: 31690790
    • Morscher RJ  et al. Mitochondrial translation requires folate-dependent tRNA methylation. Nature 554:128-132 (2018). PubMed: 29364879

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab139178.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.