Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (ab248861)

Overview

  • Product name

    Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free
    See all Ndufs4 primary antibodies
  • Description

    Rabbit monoclonal [EPR7831] to Ndufs4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IP, WB, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ndufs4 aa 50-150. The exact sequence is proprietary.

  • General notes

    Ab248861 is the carrier-free version of ab139178. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248861 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab248861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 20 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use of a HRP/AP polymerized secondary antibody is recommended.

 

  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function

      Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
    • Involvement in disease

      Defects in NDUFS4 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
    • Sequence similarities

      Belongs to the complex I NDUFS4 subunit family.
    • Cellular localization

      Mitochondrion inner membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • AQDQ antibody
      • CI 18 antibody
      • CI 18 kDa antibody
      • CI AQDQ antibody
      • CI-18 kDa antibody
      • CI-AQDQ antibody
      • Complex I 18 kDa antibody
      • Complex I AQDQ antibody
      • Complex I-18 kDa antibody
      • Complex I-AQDQ antibody
      • mitochondrial antibody
      • mitochondrial respiratory chain complex I (18 KD subunit) antibody
      • NADH coenzyme Q reductase antibody
      • NADH dehydrogenase (ubiquinone) Fe S protein 4 18kDa antibody
      • NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 antibody
      • NADH dehydrogenase antibody
      • NADH ubiquinone oxidoreductase 18 kDa subunit antibody
      • NADH-ubiquinone oxidoreductase 18 kDa subunit antibody
      • NDUFS4 antibody
      • NDUS4_HUMAN antibody
      see all

    References

    ab248861 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab248861.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up