Overview

  • Product name

    Anti-NDUFS5 antibody [EPR12291]
    See all NDUFS5 primary antibodies
  • Description

    Rabbit monoclonal [EPR12291] to NDUFS5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human NDUFS5. The exact sequence is proprietary.
    Database link: O43920

  • Positive control

    • Human fetal liver, Human fetal brain, HUVEC, K562 and HepG2 lysates; Human heart tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
    • Sequence similarities

      Belongs to the complex I NDUFS5 subunit family.
    • Domain

      Contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds.
    • Cellular localization

      Mitochondrion. Mitochondrion inner membrane. Mitochondrion intermembrane space.
    • Information by UniProt
    • Database links

    • Alternative names

      • CI 15k antibody
      • CI-15 kDa antibody
      • CI15K antibody
      • Complex I-15 kDa antibody
      • NADH dehydrogenase (ubiquinone) Fe S protein 5, 15kDa (NADH coenzyme Q reductase) antibody
      • NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 antibody
      • NADH-ubiquinone oxidoreductase 15 kDa subunit antibody
      • NADH:ubiquinone oxidoreductase 15 kDa IP subunit antibody
      • NDUFS5 antibody
      • NDUS5_HUMAN antibody
      see all

    Images

    • All lanes : Anti-NDUFS5 antibody [EPR12291] (ab179806) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : HUVEC lysate
      Lane 4 : K562 lysate
      Lane 5 : HepG2 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 13 kDa

    • Immunohistochemical analysis of paraffin embedded Human heart tissue labeling NDUFS5 with ab179806 at 1/50.

    References

    This product has been referenced in:

    • Habich M  et al. Vectorial Import via a Metastable Disulfide-Linked Complex Allows for a Quality Control Step and Import by the Mitochondrial Disulfide Relay. Cell Rep 26:759-774.e5 (2019). Read more (PubMed: 30650365) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab179806.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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