Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12291] to NDUFS5
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-NDUFS5 antibody [EPR12291]
See all NDUFS5 primary antibodies
DescriptionRabbit monoclonal [EPR12291] to NDUFS5
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Human
Recombinant fragment within Human NDUFS5. The exact sequence is proprietary.
Database link: O43920
- Human fetal liver, Human fetal brain, HUVEC, K562 and HepG2 lysates; Human heart tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab179806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Sequence similaritiesBelongs to the complex I NDUFS5 subunit family.
DomainContains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds.
Cellular localizationMitochondrion. Mitochondrion inner membrane. Mitochondrion intermembrane space.
- Information by UniProt
- CI 15k antibody
- CI-15 kDa antibody
- CI15K antibody
All lanes : Anti-NDUFS5 antibody [EPR12291] (ab179806) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal brain lysate
Lane 3 : HUVEC lysate
Lane 4 : K562 lysate
Lane 5 : HepG2 lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 13 kDa
Immunohistochemical analysis of paraffin embedded Human heart tissue labeling NDUFS5 with ab179806 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab179806 has been referenced in 1 publication.
- Habich M et al. Vectorial Import via a Metastable Disulfide-Linked Complex Allows for a Quality Control Step and Import by the Mitochondrial Disulfide Relay. Cell Rep 26:759-774.e5 (2019). PubMed: 30650365