Overview

  • Product name

  • Description

    Rabbit polyclonal to NDUFS8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human NDUFS8 aa 160-210. The exact sequence is proprietary.
    Database link: O00217

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab241346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 24 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone.
  • Involvement in disease

    Defects in NDUFS8 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
  • Sequence similarities

    Belongs to the complex I 23 kDa subunit family.
    Contains 2 4Fe-4S ferredoxin-type domains.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • CI 23kD antibody
    • CI-23kD antibody
    • Complex I 23kD antibody
    • Complex I-23kD antibody
    • EC 1.6.5.3 antibody
    • EC 1.6.99.3 antibody
    • Human mitochondrial NADH dehydrogenase ubiquinone Fe S protein 8 antibody
    • NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial antibody
    • NADH ubiquinone oxidoreductase 23 kDa subunit antibody
    • NADH-ubiquinone oxidoreductase 23 kDa subunit antibody
    • NDUFS8 antibody
    • NDUS8_HUMAN antibody
    • TYKY antibody
    • TYKY subunit antibody
    • Ubiquinoneiron sulfur protein 8, mitochondrial precursor antibody
    see all

Images

  • All lanes : Anti-NDUFS8 antibody (ab241346) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 24 kDa


    Exposure time: 30 seconds


    Lysates prepared using NETN lysis buffer.

  • NDUFS8 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
    ab241346 used for IP at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab241346 at 1 µg/ml.

    Lane 1: ab241346 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab241346 has not yet been referenced specifically in any publications.

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