Overview

  • Product name

    Anti-NDUFV2 antibody [EPR15351(B)] (HRP)
    See all NDUFV2 primary antibodies
  • Description

    Rabbit monoclonal [EPR15351(B)] to NDUFV2 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NDUFV2 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P19404

  • Positive control

    • WB: Human Fetal Heart tissue lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab209040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 27 kDa).

Target

  • Function

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Sequence similarities

    Belongs to the complex I 24 kDa subunit family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 24kDa subunit of Complex I antibody
    • CI-24k antibody
    • complex I 24kDa subunit antibody
    • complex I, mitochondrial respiratory 2 antibody
    • mitochondrial antibody
    • NADH dehydrogenase (ubiquinone) flavoprotein 2 antibody
    • NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa antibody
    • NADH dehydrogenase [ubiquinone] flavoprotein 2 antibody
    • NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial antibody
    • NADH ubiquinone oxidoreductase 24 kDa subunit antibody
    • NADH-ubiquinone oxidoreductase 24 kDa subunit antibody
    • NADH-ubiquinone oxidoreductase flavoprotein 2 antibody
    • NDUFV2 antibody
    • NDUV2_HUMAN antibody
    • nuclear-encoded mitochondrial NADH-ubiquinone reductase 24Kd subunit antibody
    • Ubiquinoneflavoprotein 2 antibody
    • Ubiquinoneflavoprotein 2, mitochondrial precursor antibody
    see all

Images

  • Anti-NDUFV2 antibody [EPR15351(B)] (HRP) (ab209040) at 1/5000 dilution + Heart (Human) Tissue Lysate - fetal normal tissue at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209040 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab209040 has not yet been referenced specifically in any publications.

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