Overview

  • Product name

    Anti-NEAS antibody [3D7]
    See all NEAS primary antibodies
  • Description

    Mouse monoclonal [3D7] to NEAS
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Cow, Human
  • Immunogen

    Recombinant fragment corresponding to Human NEAS.

  • Positive control

    • Rat hippocampal lysate; cultured rat neurons and glia.
  • General notes

     This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)

     

Properties

Applications

Our Abpromise guarantee covers the use of ab131575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/3000. Detects a band of approximately 240 kDa (predicted molecular weight: 285 kDa).
ICC/IF 1/500.

Target

  • Function

    Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
  • Involvement in disease

    Defects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
  • Sequence similarities

    Belongs to the spectrin family.
    Contains 3 EF-hand domains.
    Contains 1 SH3 domain.
    Contains 23 spectrin repeats.
  • Post-translational
    modifications

    Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • (ALPHA)II-SPECTRIN antibody
    • Alpha II Spectrin antibody
    • Alpha-II spectrin antibody
    • brain antibody
    • EIEE5 antibody
    • FLJ17738 antibody
    • FLJ44613 antibody
    • Fodrin alpha chain antibody
    • Fodrin, alpha antibody
    • NEAS antibody
    • Non erythrocytic spectrin alpha antibody
    • non-erythroid alpha chain antibody
    • SPECA antibody
    • Spectrin alpha chain antibody
    • Spectrin alpha chain brain antibody
    • Spectrin alpha non erythrocytic 1 antibody
    • Spectrin antibody
    • Spectrin non erythroid alpha chain antibody
    • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
    • Spectrin, nonerythroid, alpha subunit antibody
    • Spna2 antibody
    • SPTA 2 antibody
    • SPTA2 antibody
    • SPTA2_HUMAN antibody
    • SPTAN 1 antibody
    • SPTAN1 antibody
    see all

Images

  • Anti-NEAS antibody [3D7] (ab131575) at 1/3000 dilution + Rat hippocampal lysate

    Predicted band size: 285 kDa
    Observed band size: 240 kDa
    why is the actual band size different from the predicted?

  • Immunoflourescent analysis of cultured rat neurons and glia labelling NEAS with ab131575 at 1/500 dilution (green). The DNA is labelled Blue.

References

This product has been referenced in:

  • Gimenez-Molina Y  et al. The Differential Organization of F-Actin Alters the Distribution of Organelles in Cultured When Compared to Native Chromaffin Cells. Front Cell Neurosci 11:135 (2017). Read more (PubMed: 28522964) »
See 1 Publication for this product

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