Product nameAnti-NEAS antibody [D8B7]
See all NEAS primary antibodies
DescriptionMouse monoclonal [D8B7] to NEAS
Tested applicationsSuitable for: ICC/IF, IP, IHC-P, ELISA, WB, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Sheep, Human, African green monkey, Chinese hamster
Full length native protein (purified) corresponding to Human NEAS.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab11755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 21059848|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. See Abreviews.|
|ELISA||1/100000 - 1/1e+006.|
|WB||1/100 - 1/1000. Predicted molecular weight: 297 kDa.|
|IHC-Fr||1/10 - 1/50.|
FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Involvement in diseaseDefects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
Sequence similaritiesBelongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 23 spectrin repeats.
modificationsPhosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
- Information by UniProt
- (ALPHA)II-SPECTRIN antibody
- Alpha II Spectrin antibody
- Alpha-II spectrin antibody
Anti-NEAS antibody [D8B7] (ab11755) at 1/1000 dilution + 3T3 whole cell lysate at 30 µg
HRP conjugated goat anti-mouse at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 297 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
IF using ab11755.
ab11755 at 1/100 staining mouse gut (small bowel) tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.
This product has been referenced in:
- Wang Y et al. Critical roles of aII spectrin in brain development and epileptic encephalopathy. J Clin Invest 128:760-773 (2018). Read more (PubMed: 29337302) »
- Yamamoto K et al. The neuroprotective effect of latanoprost acts via klotho-mediated suppression of calpain activation after optic nerve transection. J Neurochem 140:495-508 (2017). WB . Read more (PubMed: 27859240) »