Product nameAnti-NEAS antibody [EPR3016]
See all NEAS primary antibodies
DescriptionRabbit monoclonal [EPR3016] to NEAS
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human NEAS aa 50-150. The exact sequence is proprietary.
- HeLa cell extract; Jurkat cell extract and lysate; Human colon and kidney tissue.
This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 285,150 kDa (predicted molecular weight: 285 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Involvement in diseaseDefects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
Sequence similaritiesBelongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 23 spectrin repeats.
modificationsPhosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
- Information by UniProt
- (ALPHA)II-SPECTRIN antibody
- Alpha II Spectrin antibody
- Alpha-II spectrin antibody
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human colon tissue staining NEAS with ab133342 at 1/10,000 dilution.
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human kidney tissue staining NEAS with ab133342 at 1/10,000 dilution.
All lanes : Anti-NEAS antibody [EPR3016] (ab133342) at 1/10000 dilution
Lane 1 : HeLa cells treated with Camptothecin
Lane 2 : HeLa cell extract
Lane 3 : Jurkat cells treated with Saturosporine
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 285 kDa
Observed band size: 150,285 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin embedded normal Human stomach tissue using ab133342 showing +ve staining.
Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab133342 showing +ve staining.