Overview

  • Product name

    Anti-NEAS antibody [EPR3016]
    See all NEAS primary antibodies
  • Description

    Rabbit monoclonal [EPR3016] to NEAS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NEAS aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa cell extract; Jurkat cell extract and lysate; Human colon and kidney tissue.
  • General notes

     

     

     This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 285,150 kDa (predicted molecular weight: 285 kDa).
IP 1/10.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
    • Involvement in disease

      Defects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
    • Sequence similarities

      Belongs to the spectrin family.
      Contains 3 EF-hand domains.
      Contains 1 SH3 domain.
      Contains 23 spectrin repeats.
    • Post-translational
      modifications

      Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • (ALPHA)II-SPECTRIN antibody
      • Alpha II Spectrin antibody
      • Alpha-II spectrin antibody
      • brain antibody
      • EIEE5 antibody
      • FLJ17738 antibody
      • FLJ44613 antibody
      • Fodrin alpha chain antibody
      • Fodrin, alpha antibody
      • NEAS antibody
      • Non erythrocytic spectrin alpha antibody
      • non-erythroid alpha chain antibody
      • SPECA antibody
      • Spectrin alpha chain antibody
      • Spectrin alpha chain brain antibody
      • Spectrin alpha non erythrocytic 1 antibody
      • Spectrin antibody
      • Spectrin non erythroid alpha chain antibody
      • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
      • Spectrin, nonerythroid, alpha subunit antibody
      • Spna2 antibody
      • SPTA 2 antibody
      • SPTA2 antibody
      • SPTA2_HUMAN antibody
      • SPTAN 1 antibody
      • SPTAN1 antibody
      see all

    Images

    • Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human colon tissue staining NEAS with ab133342 at 1/10,000 dilution.

    • Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human kidney tissue staining NEAS with ab133342 at 1/10,000 dilution.

    • All lanes : Anti-NEAS antibody [EPR3016] (ab133342) at 1/10000 dilution

      Lane 1 : HeLa cells treated with Camptothecin
      Lane 2 : HeLa cell extract
      Lane 3 : Jurkat cells treated with Saturosporine
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 285 kDa
      Observed band size: 150,285 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin embedded normal Human stomach tissue using ab133342 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab133342 showing +ve staining.

    References

    This product has been referenced in:

    • Malty RH  et al. A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling. Cell Syst 5:564-577.e12 (2017). Read more (PubMed: 29128334) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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