Application
Western blot
Sample
Human Cell lysate - whole cell (JURKAT)
Loading amount
10 µg
Specification
JURKAT
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBST+5%Milk
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/16000
Detection
Detection method
ECL Prime
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 285 kDa
Positive control
JURKAT
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Sep 05 2011