Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat, Human ATL cell lines (KK1, KOB))
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Jurkat, Human ATL cell lines (KK1, KOB)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Blocking agent
Dilution
1/2000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: HRP Goat-anti-Rabbit antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 280 and 60 kDa
Additional data
Additional Notes
The 60 kDa specific bands from 2 cell lines are unknown. (novel cleaved form?)
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Submitted Oct 26 2011