Overview

  • Product name

    Anti-NEAS antibody [EPR3830-43]
    See all NEAS primary antibodies
  • Description

    Rabbit monoclonal [EPR3830-43] to NEAS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human NEAS aa 1150-1250.

  • Positive control

    • HeLa and Jurkat cell lysate; Human breast carcinoma and colon tissues; HeLa cells.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3830-43
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
WB 1/10000 - 1/50000. Detects a band of approximately 150, 285 kDa (predicted molecular weight: 285 kDa).
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
    • Involvement in disease

      Defects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
    • Sequence similarities

      Belongs to the spectrin family.
      Contains 3 EF-hand domains.
      Contains 1 SH3 domain.
      Contains 23 spectrin repeats.
    • Post-translational
      modifications

      Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • (ALPHA)II-SPECTRIN antibody
      • Alpha II Spectrin antibody
      • Alpha-II spectrin antibody
      • brain antibody
      • EIEE5 antibody
      • FLJ17738 antibody
      • FLJ44613 antibody
      • Fodrin alpha chain antibody
      • Fodrin, alpha antibody
      • NEAS antibody
      • Non erythrocytic spectrin alpha antibody
      • non-erythroid alpha chain antibody
      • SPECA antibody
      • Spectrin alpha chain antibody
      • Spectrin alpha chain brain antibody
      • Spectrin alpha non erythrocytic 1 antibody
      • Spectrin antibody
      • Spectrin non erythroid alpha chain antibody
      • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
      • Spectrin, nonerythroid, alpha subunit antibody
      • Spna2 antibody
      • SPTA 2 antibody
      • SPTA2 antibody
      • SPTA2_HUMAN antibody
      • SPTAN 1 antibody
      • SPTAN1 antibody
      see all

    Images

    • All lanes : Anti-NEAS antibody [EPR3830-43] (ab133320) at 1/10000 dilution

      Lane 1 : HeLa cell lysate + camptothecin
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate + staurosporine
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 285 kDa

    • Immunohistochemical analysis of Paraffin Embedded Human breast carcinoma tissue labelling NEAS with ab133320 at 1/500 dilution.

    • Immunohistochemical analysis of Paraffin Embedded Human colon tissue labelling NEAS with ab133320 at 1/500 dilution.

    • Immunofluorescent analysis of HeLa cells labelling NEAS with ab133320 at 1/250 dilution.

    References

    ab133320 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133320.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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