Product nameAnti-NEAS antibody [EPR3830-43]
See all NEAS primary antibodies
DescriptionRabbit monoclonal [EPR3830-43] to NEAS
Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Rat, Human
Synthetic peptide corresponding to Human NEAS aa 1150-1250.
- HeLa and Jurkat cell lysate; Human breast carcinoma and colon tissues; HeLa cells.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab133320 in the following tested applications.
|ICC/IF||1/250 - 1/500.|
|WB||1/10000 - 1/50000. Detects a band of approximately 150, 285 kDa (predicted molecular weight: 285 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Involvement in diseaseDefects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
Sequence similaritiesBelongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 23 spectrin repeats.
modificationsPhosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
- Information by UniProt
- (ALPHA)II-SPECTRIN antibody
- Alpha II Spectrin antibody
- Alpha-II spectrin antibody
All lanes : Anti-NEAS antibody [EPR3830-43] (ab133320) at 1/10000 dilution
Lane 1 : HeLa cell lysate + camptothecin
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate + staurosporine
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 285 kDa
Immunohistochemical analysis of Paraffin Embedded Human breast carcinoma tissue labelling NEAS with ab133320 at 1/500 dilution.
Immunohistochemical analysis of Paraffin Embedded Human colon tissue labelling NEAS with ab133320 at 1/500 dilution.
Immunofluorescent analysis of HeLa cells labelling NEAS with ab133320 at 1/250 dilution.
ab133320 has not yet been referenced specifically in any publications.