ICPs & late structural (virion) antigens. Cross reactivity is >90% with human rotaviruses (reported). Uninfected cell reactivity is negative against HEp-2 cells and WI-38 cells by indirect immunofluorescence.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF: 1/10 - 1/50.
Acetone fixation of the antigen source is recommended prior to staining.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Bovine virus diarrhea (BVD) is a complicated disease to discuss as it can result in a wide variety of disease problems from very mild to very severe. BVD can be one of the most devastating diseases cattle encounter and one of the hardest to get rid of when it attacks a herd. The viruses that cause BVD have been grouped into two genotypes, Type I and Type II. The disease syndrome caused by the two genotypes is basically the same, however disease caused by Type II infection is often more severe. The various disease syndromes noted in cattle infected with BVD virus are mainly attributed to the age of the animal when it became infected and to certain characteristics of the virus involved.