Key features and details
- Mouse monoclonal [CL0575] to NECAB1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-NECAB1 antibody [CL0575]
See all NECAB1 primary antibodies
DescriptionMouse monoclonal [CL0575] to NECAB1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Orangutan
EpitopeBinds to an epitope located within the peptidesequence SIQWPGKRSS as determined by overlapping synthetic peptides.
- IHC-P: Human cerebral cotex and kidney tissues. WB: Human cerebral cortex lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab244217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa.|
Tissue specificityExpressed in brain (at protein level).
Sequence similaritiesContains 1 ABM domain.
Contains 2 EF-hand domains.
- Information by UniProt
- EF-hand calcium-binding protein 1 antibody
- EFCBP1 antibody
- N-terminal EF-hand calcium-binding protein 1 antibody
Paraffin-embedded human cerebral cortex tissue stained for NECAB1 using ab244217 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for NECAB1 using ab244217 at 1/500 dilution in immunohistochemical analysis.
Anti-NECAB1 antibody [CL0575] (ab244217) at 1 µg/ml + Human cerebral cortex lysate
Predicted band size: 41 kDa
Paraffin-embedded human liver tissue stained for NECAB1 using ab244217 at 1/500 dilution in immunohistochemical analysis. Negative control.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244217 has not yet been referenced specifically in any publications.