Recombinant Anti-NECAP2 antibody [EPR11744] (ab175210)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11744] to NECAP2
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NECAP2 antibody [EPR11744]
See all NECAP2 primary antibodies -
Description
Rabbit monoclonal [EPR11744] to NECAP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human NECAP2 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NVZ3 -
Positive control
- Human fetal kidney, fetal liver tissue lysate and C6, RAW 264.7, PC 12 and NIH 3T3 cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11744 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 28 kDa.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 28 kDa. |
IP
1/10 - 1/100. |
Target
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Function
Involved in endocytosis. -
Sequence similarities
Belongs to the NECAP family. -
Domain
The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself. -
Cellular localization
Cytoplasmic vesicle, clathrin-coated vesicle membrane. Cell membrane. Colocalizes with AP-2 at the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 55707 Human
- Entrez Gene: 66147 Mouse
- Entrez Gene: 298598 Rat
- Omim: 611624 Human
- SwissProt: Q9NVZ3 Human
- SwissProt: Q9D1J1 Mouse
- SwissProt: Q6P756 Rat
- Unigene: 437385 Human
see all -
Alternative names
- Adaptin ear binding coat associated protein 2 antibody
- Adaptin ear-binding coat-associated protein 2 antibody
- FLJ10420 antibody
see all
Images
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All lanes : Anti-NECAP2 antibody [EPR11744] (ab175210) at 1/1000 dilution
Lane 1 : Fetal kidney tissue lysate
Lane 2 : Fetal liver tissue lysate
Lane 3 : C6 cell lysate
Lane 4 : RAW 264.7 cell lysate
Lane 5 : PC 12 cell lysate
Lane 6 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 28 kDa -
Western blot analysis on immunoprecipitation pellet from fetal liver tissue lysate (lane 1) labeling NECAP2 with ab175210 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab175210 has not yet been referenced specifically in any publications.