Overview

  • Product name
    Anti-NECAP2 antibody [EPR11744]
  • Description
    Rabbit monoclonal [EPR11744] to NECAP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NECAP2 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NVZ3

  • Positive control
    • Human fetal kidney, fetal liver tissue lysate and C6, RAW 264.7, PC 12 and NIH 3T3 cell lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11744
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 28 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Involved in endocytosis.
    • Sequence similarities
      Belongs to the NECAP family.
    • Domain
      The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself.
    • Cellular localization
      Cytoplasmic vesicle, clathrin-coated vesicle membrane. Cell membrane. Colocalizes with AP-2 at the plasma membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adaptin ear binding coat associated protein 2 antibody
      • Adaptin ear-binding coat-associated protein 2 antibody
      • FLJ10420 antibody
      • NECAP endocytosis associated 2 antibody
      • NECAP endocytosis associated protein 2 antibody
      • NECAP endocytosis-associated protein 2 antibody
      • NECAP-2 antibody
      • NECAP2 antibody
      • NECP2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-NECAP2 antibody [EPR11744] (ab175210) at 1/1000 dilution

      Lane 1 : Fetal kidney tissue lysate
      Lane 2 : Fetal liver tissue lysate
      Lane 3 : C6 cell lysate
      Lane 4 : RAW 264.7 cell lysate
      Lane 5 : PC 12 cell lysate
      Lane 6 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 28 kDa

    • Western blot analysis on immunoprecipitation pellet from fetal liver tissue lysate (lane 1) labeling NECAP2 with ab175210 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

    References

    ab175210 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab175210.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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