Recombinant
RabMAb

Recombinant Anti-NECAP2 antibody [EPR11744] (ab175210)

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Overview

  • Product name

    Anti-NECAP2 antibody [EPR11744]
    See all NECAP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR11744] to NECAP2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human NECAP2 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NVZ3

  • Positive control

    • Human fetal kidney, fetal liver tissue lysate and C6, RAW 264.7, PC 12 and NIH 3T3 cell lysate

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11744

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 28 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Function

      Involved in endocytosis.

    • Sequence similarities

      Belongs to the NECAP family.

    • Domain

      The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself.

    • Cellular localization

      Cytoplasmic vesicle, clathrin-coated vesicle membrane. Cell membrane. Colocalizes with AP-2 at the plasma membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Adaptin ear binding coat associated protein 2 antibody
      • Adaptin ear-binding coat-associated protein 2 antibody
      • FLJ10420 antibody
      • NECAP endocytosis associated 2 antibody
      • NECAP endocytosis associated protein 2 antibody
      • NECAP endocytosis-associated protein 2 antibody
      • NECAP-2 antibody
      • NECAP2 antibody
      • NECP2_HUMAN antibody
      see all

    Images

    • Western blot - Anti-NECAP2 antibody [EPR11744] (ab175210)
      Western blot - Anti-NECAP2 antibody [EPR11744] (ab175210)
      All lanes : Anti-NECAP2 antibody [EPR11744] (ab175210) at 1/1000 dilution

      Lane 1 : Fetal kidney tissue lysate
      Lane 2 : Fetal liver tissue lysate
      Lane 3 : C6 cell lysate
      Lane 4 : RAW 264.7 cell lysate
      Lane 5 : PC 12 cell lysate
      Lane 6 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 28 kDa

    • Immunoprecipitation - Anti-NECAP2 antibody [EPR11744] (ab175210)
      Immunoprecipitation - Anti-NECAP2 antibody [EPR11744] (ab175210)

      Western blot analysis on immunoprecipitation pellet from fetal liver tissue lysate (lane 1) labeling NECAP2 with ab175210 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

    References

    ab175210 has not yet been referenced specifically in any publications.

    Publishing research using ab175210? Please let us know so that we can cite the reference in this datasheet.

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