RecombinantRabMAb

Recombinant Anti-NECAP2 antibody [EPR11744] (ab175210)

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Western blot - Anti-NECAP2 antibody [EPR11744] (ab175210)
  • Immunoprecipitation - Anti-NECAP2 antibody [EPR11744] (ab175210)
  • Anti-NECAP2 antibody [EPR11744] (ab175210)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11744] to NECAP2
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NECAP2 antibody [EPR11744]
    See all NECAP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR11744] to NECAP2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human NECAP2 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NVZ3

  • Positive control

    • Human fetal kidney, fetal liver tissue lysate and C6, RAW 264.7, PC 12 and NIH 3T3 cell lysate

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11744

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 28 kDa.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 28 kDa.
IP
1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Function

      Involved in endocytosis.

    • Sequence similarities

      Belongs to the NECAP family.

    • Domain

      The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself.

    • Cellular localization

      Cytoplasmic vesicle, clathrin-coated vesicle membrane. Cell membrane. Colocalizes with AP-2 at the plasma membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Adaptin ear binding coat associated protein 2 antibody
      • Adaptin ear-binding coat-associated protein 2 antibody
      • FLJ10420 antibody
      • NECAP endocytosis associated 2 antibody
      • NECAP endocytosis associated protein 2 antibody
      • NECAP endocytosis-associated protein 2 antibody
      • NECAP-2 antibody
      • NECAP2 antibody
      • NECP2_HUMAN antibody
      see all

    Images

    • Western blot - Anti-NECAP2 antibody [EPR11744] (ab175210)
      Western blot - Anti-NECAP2 antibody [EPR11744] (ab175210)
      All lanes : Anti-NECAP2 antibody [EPR11744] (ab175210) at 1/1000 dilution

      Lane 1 : Fetal kidney tissue lysate
      Lane 2 : Fetal liver tissue lysate
      Lane 3 : C6 cell lysate
      Lane 4 : RAW 264.7 cell lysate
      Lane 5 : PC 12 cell lysate
      Lane 6 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 28 kDa

    • Immunoprecipitation - Anti-NECAP2 antibody [EPR11744] (ab175210)
      Immunoprecipitation - Anti-NECAP2 antibody [EPR11744] (ab175210)

      Western blot analysis on immunoprecipitation pellet from fetal liver tissue lysate (lane 1) labeling NECAP2 with ab175210 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

    • Anti-NECAP2 antibody [EPR11744] (ab175210)
      Anti-NECAP2 antibody [EPR11744] (ab175210)

    References (0)

    Publishing research using ab175210? Please let us know so that we can cite the reference in this datasheet.

    ab175210 has not yet been referenced specifically in any publications.

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