Anti-NECAP2 antibody [EPR11744] (ab175210)
- Datasheet
- References
- Protocols
Overview
-
Product nameAnti-NECAP2 antibody [EPR11744]
See all NECAP2 primary antibodies -
DescriptionRabbit monoclonal [EPR11744] to NECAP2
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide within Human NECAP2 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NVZ3 -
Positive control
- Human fetal kidney, fetal liver tissue lysate and C6, RAW 264.7, PC 12 and NIH 3T3 cell lysate
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR11744
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab175210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 28 kDa. | |
| IP | 1/10 - 1/100. |
Target
-
FunctionInvolved in endocytosis.
-
Sequence similaritiesBelongs to the NECAP family.
-
DomainThe WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself.
-
Cellular localizationCytoplasmic vesicle, clathrin-coated vesicle membrane. Cell membrane. Colocalizes with AP-2 at the plasma membrane.
- Information by UniProt
-
Database links
- Entrez Gene: 55707 Human
- Entrez Gene: 66147 Mouse
- Entrez Gene: 298598 Rat
- Omim: 611624 Human
- SwissProt: Q9NVZ3 Human
- SwissProt: Q9D1J1 Mouse
- SwissProt: Q6P756 Rat
- Unigene: 437385 Human
see all -
Alternative names
- Adaptin ear binding coat associated protein 2 antibody
- Adaptin ear-binding coat-associated protein 2 antibody
- FLJ10420 antibody
see all
Images
-
![Western blot - Anti-NECAP2 antibody [EPR11744] (ab175210)](https://www.abcam.com/ps/products/175/ab175210/Images/ab175210-201713-ab1752101.JPG)
Western blot - Anti-NECAP2 antibody [EPR11744] (ab175210)All lanes : Anti-NECAP2 antibody [EPR11744] (ab175210) at 1/1000 dilution
Lane 1 : Fetal kidney tissue lysate
Lane 2 : Fetal liver tissue lysate
Lane 3 : C6 cell lysate
Lane 4 : RAW 264.7 cell lysate
Lane 5 : PC 12 cell lysate
Lane 6 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 28 kDa -
![Immunoprecipitation - Anti-NECAP2 antibody [EPR11744] (ab175210)](https://www.abcam.com/ps/products/175/ab175210/Images/ab175210-201714-ab1752102.JPG)
Immunoprecipitation - Anti-NECAP2 antibody [EPR11744] (ab175210)Western blot analysis on immunoprecipitation pellet from fetal liver tissue lysate (lane 1) labeling NECAP2 with ab175210 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.
Datasheets and documents
References
ab175210 has not yet been referenced specifically in any publications.
