Recombinant
RabMAb

Recombinant Anti-NECAP2 antibody [EPR11744] - BSA and Azide free (ab249863)

Overview

  • Product name
    Anti-NECAP2 antibody [EPR11744] - BSA and Azide free
    See all NECAP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR11744] to NECAP2 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NECAP2 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NVZ3

  • General notes

    ab249863 is a PBS-only buffer format of ab175210. Please refer to ab175210 for recommended dilutions, protocols, and image data.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Affinity purified
  • Clonality
    Monoclonal
  • Clone number
    EPR11744
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab249863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Involved in endocytosis.
    • Sequence similarities
      Belongs to the NECAP family.
    • Domain
      The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself.
    • Cellular localization
      Cytoplasmic vesicle, clathrin-coated vesicle membrane. Cell membrane. Colocalizes with AP-2 at the plasma membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adaptin ear binding coat associated protein 2 antibody
      • Adaptin ear-binding coat-associated protein 2 antibody
      • FLJ10420 antibody
      • NECAP endocytosis associated 2 antibody
      • NECAP endocytosis associated protein 2 antibody
      • NECAP endocytosis-associated protein 2 antibody
      • NECAP-2 antibody
      • NECAP2 antibody
      • NECP2_HUMAN antibody
      see all

    References

    ab249863 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab249863.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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