Overview

  • Product name
  • Description
    Rabbit polyclonal to Necdin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein (GST-tag) corresponding to Human Necdin aa 1-143 (N terminal).

  • Positive control
    • Jurkat lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa). Non-specific proteins were also detected at 70 and 100kDa, detection of which was inhibited by preincubation with the immunizing peptide.
ChIP Use at an assay dependent dilution. PubMed: 20663865

Target

  • Function
    Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.
  • Tissue specificity
    Almost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leukocytes. In brain, restricted to post-mitotic neurons.
  • Sequence similarities
    Contains 1 MAGE domain.
  • Cellular localization
    Perikaryon. Nucleus. Neural perikarya, translocates to the nucleus of postmitotic neurons and interacts with the nuclear matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • HsT16328 antibody
    • NDN antibody
    • NECD_HUMAN antibody
    • Necdin antibody
    • Necdin homolog antibody
    • Necdin MAGE family member antibody
    • necdin related protein antibody
    • PWCR antibody
    see all

Images

  • Jurkat cell lysate was probed with ab18554 at 1µg/ml.

References

This product has been referenced in:
  • Wu M  et al. The ciliary protein cystin forms a regulatory complex with necdin to modulate Myc expression. PLoS One 8:e83062 (2013). ChIP . Read more (PubMed: 24349431) »
  • Haviland R  et al. Necdin, a Negative Growth Regulator, Is a Novel STAT3 Target Gene Down-Regulated in Human Cancer. PLoS One 6:e24923 (2011). WB ; Human . Read more (PubMed: 22046235) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issuedtwo free of charge replacements:

One vial of ab18554 is being replaced with one vial of ab9574.

One vial of ab70303 is being replaced with one vial of ab126765.


To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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