Overview

  • Product name

  • Description

    Rabbit polyclonal to NECL-1
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of total NECL-1 protein.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human NECL-1 (internal sequence).

  • Positive control

    • Extracts from RAW264.7 and NIH-3T3 cells
  • General notes

    Previously labelled as CADM3. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
ELISA 1/5000.

Target

  • Function

    Involved in the cell-cell adhesion. Has both calcium-independent homophilic cell-cell adhesion activity and calcium-independent heterophilic cell-cell adhesion activity with IGSF4, PVRL1 and PVRL3. Interaction with EPB41L1 may regulate structure or function of cell-cell junctions.
  • Tissue specificity

    Isoform 1 is expressed mainly in adult and fetal brain. Isoform 2 is highly expressed in adult brain and weakly expressed in placenta. In brain, Isoform 2 is highly expressed in cerebellum.
  • Sequence similarities

    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Domain

    The cytoplasmic region mediates interaction with EPB41L1, DLG3, MPP6 and CASK.
  • Cellular localization

    Cell junction. Cell membrane. Localized at the cell-cell attached sites of the plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BIgR antibody
    • Brain immunoglobulin receptor antibody
    • CADM 3 antibody
    • cadm3 antibody
    • CADM3_HUMAN antibody
    • Cell adhesion molecule 3 antibody
    • Dendritic cell nectin like protein 1 short isoform antibody
    • FLJ10698 antibody
    • IgSF4B antibody
    • Immunoglobulin superfamily member 4B antibody
    • Necl 1 antibody
    • Necl-1 antibody
    • NECL1 antibody
    • Nectin like 1 antibody
    • Nectin like protein 1 antibody
    • Nectin-like protein 1 antibody
    • Synaptic cell adhesion molecule 3 antibody
    • synCAM3 antibody
    • TSLC1 like 1 antibody
    • TSLC1 like protein 1 antibody
    • TSLC1-like protein 1 antibody
    • TSLL1 antibody
    see all

Images

  • All lanes : Anti-NECL-1 antibody (ab69604) at 1/500 dilution

    Lane 1 : extracts from RAW264.7 cells
    Lane 2 : extracts from NIH-3T3 cells
    Lane 3 : extracts from NIH-3T3 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

References

ab69604 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab69604.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up