Necrostatin-1, necroptosis inhibitor (ab141053)
Key features and details
- Potent, specific necroptosis inhibitor
- CAS Number: 4311-88-0
- Purity: > 99%
- Soluble in ethanol to 50 mM and in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Necrostatin-1, necroptosis inhibitor -
Description
Potent, specific necroptosis inhibitor -
Biological description
Potent, specific necroptosis inhibitor (EC50 = 490 nM). Selective allosteric inhibitor of death domain receptor-associated adaptor kinase RIP1 in vitro (EC50 = 182 nM). Caspase independent, programmed necrosis pathway.
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Purity
> 99% -
CAS Number
4311-88-0 -
Chemical structure
Properties
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Chemical name
5-(1H-Indol-3-ylmethyl)-3-methyl-2-thioxo-4-imidazolidinone -
Molecular weight
259.33 -
Molecular formula
C13H13N3OS -
PubChem identifier
2828334 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in ethanol to 50 mM and in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
S=C3NC(Cc1cnc2ccccc12)C(=O)N3C -
Source
Synthetic
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Research areas
Images
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Chemical Structure - Necrostatin-1, necroptosis inhibitor (ab141053)2D chemical structure image of ab141053, Necrostatin-1, necroptosis inhibitor
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Functional Studies - Necrostatin-1, necroptosis inhibitor (ab141053)Yan et al PLoS One. 2018 Nov 21;13(11):e0207760. doi: 10.1371/journal.pone.0207760. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Necroptosis exists in PDLSCs.
TEM, a representative normal cell (the cell had a long fusiform, cell membrane structure was complete, the nucleus was round, nucleolus was obvious and center), b representative necroptosis cell (cell membrane ruptured, organelle disappeared, chromatin consolidated and agglutinated, presenting necrotic ultrastructural characteristics), c representative necrosis cell after Nec-1 inhibited (the cell membrane was intact, and the chromatin structure returned to normal).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (12)
ab141053 has been referenced in 12 publications.
- Molnár T et al. Caspase-9 acts as a regulator of necroptotic cell death. FEBS J 288:6476-6491 (2021). PubMed: 33899329
- Lau H et al. Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets. Int J Mol Sci 22:N/A (2021). PubMed: 34445075
- Trimova G et al. Tumour necrosis factor alpha promotes secretion of 14-3-3? by inducing necroptosis in macrophages. Arthritis Res Ther 22:24 (2020). PubMed: 32051018
- Alegre-Cortés E et al. Toxicity of Necrostatin-1 in Parkinson's Disease Models. Antioxidants (Basel) 9:N/A (2020). PubMed: 32549347
- Lau H et al. Dose-dependent effects of necrostatin-1 supplementation to tissue culture media of young porcine islets. PLoS One 15:e0243506 (2020). PubMed: 33284818
