Necrostatin-1, necroptosis inhibitor (ab141053)
Key features and details
- Potent, specific necroptosis inhibitor
- CAS Number: 4311-88-0
- Purity: > 99%
- Soluble in ethanol to 50 mM and in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
-
Product name
Necrostatin-1, necroptosis inhibitor -
Description
Potent, specific necroptosis inhibitor -
Biological description
Potent, specific necroptosis inhibitor (EC50 = 490 nM). Selective allosteric inhibitor of death domain receptor-associated adaptor kinase RIP1 in vitro (EC50 = 182 nM). Caspase independent, programmed necrosis pathway.
-
Purity
> 99% -
CAS Number
4311-88-0 -
Chemical structure
Properties
-
Chemical name
5-(1H-Indol-3-ylmethyl)-3-methyl-2-thioxo-4-imidazolidinone -
Molecular weight
259.33 -
Molecular formula
C13H13N3OS -
PubChem identifier
2828334 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in ethanol to 50 mM and in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
SMILES
S=C3NC(Cc1cnc2ccccc12)C(=O)N3C -
Source
Synthetic
-
Research areas
Images
-
Chemical Structure - Necrostatin-1, necroptosis inhibitor (ab141053)2D chemical structure image of ab141053, Necrostatin-1, necroptosis inhibitor
-
Functional Studies - Necrostatin-1, necroptosis inhibitor (ab141053)Yan et al PLoS One. 2018 Nov 21;13(11):e0207760. doi: 10.1371/journal.pone.0207760. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Necroptosis exists in PDLSCs.
TEM, a representative normal cell (the cell had a long fusiform, cell membrane structure was complete, the nucleus was round, nucleolus was obvious and center), b representative necroptosis cell (cell membrane ruptured, organelle disappeared, chromatin consolidated and agglutinated, presenting necrotic ultrastructural characteristics), c representative necrosis cell after Nec-1 inhibited (the cell membrane was intact, and the chromatin structure returned to normal).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (9)
ab141053 has been referenced in 9 publications.
- Trimova G et al. Tumour necrosis factor alpha promotes secretion of 14-3-3? by inducing necroptosis in macrophages. Arthritis Res Ther 22:24 (2020). PubMed: 32051018
- Alegre-Cortés E et al. Toxicity of Necrostatin-1 in Parkinson's Disease Models. Antioxidants (Basel) 9:N/A (2020). PubMed: 32549347
- Lau H et al. Necrostatin-1 supplementation enhances young porcine islet maturation and in vitro function. Xenotransplantation N/A:e12555 (2019). PubMed: 31532037
- Bockerstett KA et al. Interleukin-17A Promotes Parietal Cell Atrophy by Inducing Apoptosis. Cell Mol Gastroenterol Hepatol 5:678-690.e1 (2018). PubMed: 29930985
- Yan B et al. Necrostatin-1 promotes ectopic periodontal tissue like structure regeneration in LPS-treated PDLSCs. PLoS One 13:e0207760 (2018). PubMed: 30462730
- Matsuda H et al. HCaRG/COMMD5 inhibits ErbB receptor-driven renal cell carcinoma. Oncotarget 8:69559-69576 (2017). PubMed: 29050225
- Tsui KH et al. DHEA protects mitochondria against dual modes of apoptosis and necroptosis in human granulosa HO23 cells. Reproduction 154:101-110 (2017). PubMed: 28624766
- Dallenga T et al. M. tuberculosis-Induced Necrosis of Infected Neutrophils Promotes Bacterial Growth Following Phagocytosis by Macrophages. Cell Host Microbe 22:519-530.e3 (2017). PubMed: 29024644
- Li CJ et al. Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis. Sci Rep 5:9819 (2015). PubMed: 25909282
