Anti-Nectin 2 antibody (ab233085)
Key features and details
- Rabbit polyclonal to Nectin 2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Nectin 2 antibody
See all Nectin 2 primary antibodies -
Description
Rabbit polyclonal to Nectin 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human Nectin 2 aa 50-400. Expressed in E. coli. N-terminal tags.
Database link: Q92692 -
Positive control
- WB: Recombinant human Nectin 2 protein; K562 and HEK-293T whole cell lysate; Mouse testis tissue lysate. IHC-P: Human kidney tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 58 kDa.
|
|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
Notes |
---|
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 58 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
-
Function
Probable cell adhesion protein. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 5819 Human
- Entrez Gene: 19294 Mouse
- Omim: 600798 Human
- SwissProt: Q92692 Human
- SwissProt: P32507 Mouse
- Unigene: 655455 Human
- Unigene: 4341 Mouse
-
Alternative names
- CD 112 antibody
- CD112 antibody
- CD112 antigen antibody
see all
Images
-
Formalin-fixed, paraffin-embedded human kidney tissue stained for Nectin 2 using ab233085 in at 20 μg/ml immunohistochemical analysis. DAB staining.
-
Anti-Nectin 2 antibody (ab233085) at 2 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml
Predicted band size: 58 kDa -
Anti-Nectin 2 antibody (ab233085) at 2 µg/ml + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml
Predicted band size: 58 kDa -
Anti-Nectin 2 antibody (ab233085) at 2 µg/ml + Mouse testis tissue lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml
Predicted band size: 58 kDa -
Anti-Nectin 2 antibody (ab233085) at 2 µg/ml + Recombinant human Nectin 2 protein
Predicted band size: 58 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab233085 has not yet been referenced specifically in any publications.