Overview

  • Product name

    Anti-Nectin 2 antibody [B-C12]
    See all Nectin 2 primary antibodies
  • Description

    Mouse monoclonal [B-C12] to Nectin 2
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    KG-1 cell line

  • Positive control

    • KG-1 cell line
  • General notes

    This product was changed from ascites to tissue culture supernatant on 4th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab47171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 10 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Probable cell adhesion protein.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 112 antibody
    • CD112 antibody
    • CD112 antigen antibody
    • Herpes virus entry mediator B antibody
    • Herpes virus entry protein B antibody
    • Herpesvirus entry mediator B antibody
    • Herpesvirus entry protein B antibody
    • Hve B antibody
    • HveB antibody
    • Nectin-2 antibody
    • Nectin2 antibody
    • Poliovirus receptor like 2 antibody
    • Poliovirus receptor related 2 (herpesvirus entry mediator B) antibody
    • Poliovirus receptor related 2 antibody
    • Poliovirus receptor related protein 2 antibody
    • Poliovirus receptor-related protein 2 antibody
    • PRR 2 antibody
    • PRR2 antibody
    • PVRL 2 antibody
    • PVRL2 antibody
    • PVRL2_HUMAN antibody
    • PVRR 2 antibody
    • PVRR2 antibody
    see all

Images

  • A typical staining pattern with ab47171 monoclonal antibody of KG-1 cell line.

    This image was generated using the ascites version of the product.

  • ICC/IF image of ab47171 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47171, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This image was generated using the ascites version of the product.

References

This product has been referenced in:

  • Ogbomo H  et al. Myxoma virus infection promotes NK lysis of malignant gliomas in vitro and in vivo. PLoS One 8:e66825 (2013). Flow Cyt ; Human . Read more (PubMed: 23762498) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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