Recombinant
RabMAb

Recombinant Anti-Nectin 2 antibody [EPR21124] - BSA and Azide free (ab239346)

Overview

  • Product name

    Anti-Nectin 2 antibody [EPR21124] - BSA and Azide free
    See all Nectin 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR21124] to Nectin 2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Nectin 2 aa 1-350. The exact sequence is proprietary.
    Database link: Q92692

  • Positive control

    • IHC-P: Human testis tissue.
  • General notes

    Ab239346 is the carrier-free version of ab233384. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239346 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 50-85 kDa (predicted molecular weight: 58 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Probable cell adhesion protein.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 112 antibody
    • CD112 antibody
    • CD112 antigen antibody
    • Herpes virus entry mediator B antibody
    • Herpes virus entry protein B antibody
    • Herpesvirus entry mediator B antibody
    • Herpesvirus entry protein B antibody
    • Hve B antibody
    • HveB antibody
    • Nectin-2 antibody
    • Nectin2 antibody
    • Poliovirus receptor like 2 antibody
    • Poliovirus receptor related 2 (herpesvirus entry mediator B) antibody
    • Poliovirus receptor related 2 antibody
    • Poliovirus receptor related protein 2 antibody
    • Poliovirus receptor-related protein 2 antibody
    • PRR 2 antibody
    • PRR2 antibody
    • PVRL 2 antibody
    • PVRL2 antibody
    • PVRL2_HUMAN antibody
    • PVRR 2 antibody
    • PVRR2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Nectin 2 with ab233384 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in human liver (PMID: 23758976) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233384).

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Nectin 2 with ab233384 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in human breast cancer (PMID: 23758976) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233384).

  • Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Nectin 2 with ab233384 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in human testis (PMID: 23758976) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233384).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab239346 has not yet been referenced specifically in any publications.

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