Overview

  • Product name

    Anti-Nectin 2 antibody [EPR6717]
    See all Nectin 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6717] to Nectin 2
  • Host species

    Rabbit
  • Specificity

    ab135246 does not recognize Isoform Alpha of human Nectin-2.
  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Nectin 2 aa 500-600 (intracellular). The exact sequence is proprietary.

  • Positive control

    • K562, 293T, T-24 and SW480 cell lysates; SW480 and HT-29 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab135246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IP 1/100 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Probable cell adhesion protein.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the nectin family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD 112 antibody
      • CD112 antibody
      • CD112 antigen antibody
      • Herpes virus entry mediator B antibody
      • Herpes virus entry protein B antibody
      • Herpesvirus entry mediator B antibody
      • Herpesvirus entry protein B antibody
      • Hve B antibody
      • HveB antibody
      • Nectin-2 antibody
      • Nectin2 antibody
      • Poliovirus receptor like 2 antibody
      • Poliovirus receptor related 2 (herpesvirus entry mediator B) antibody
      • Poliovirus receptor related 2 antibody
      • Poliovirus receptor related protein 2 antibody
      • Poliovirus receptor-related protein 2 antibody
      • PRR 2 antibody
      • PRR2 antibody
      • PVRL 2 antibody
      • PVRL2 antibody
      • PVRL2_HUMAN antibody
      • PVRR 2 antibody
      • PVRR2 antibody
      see all

    Images

    • Overlay histogram showing HepG2 cells stained with ab135246 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab135246, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Immunocytochemistry/Immunofluorescence analysis of HT-29 (Human colorectal adenocarcinoma cell line) labeling Nectin 2 with purified ab135246 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • All lanes : Anti-Nectin 2 antibody [EPR6717] (ab135246) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : ECV 304 cell lysate
      Lane 4 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 58 kDa

    • Immunofluorescent sanalysis of SW480 cells labelling Nectin 2 with ab135246 at 1/250 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Tetzlaff F  et al. MPDZ promotes DLL4-induced Notch signaling during angiogenesis. Elife 7:N/A (2018). Read more (PubMed: 29620522) »
    • Iizuka-Hishikawa Y  et al. Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis. J Biol Chem 292:12065-12076 (2017). WB ; Mouse . Read more (PubMed: 28578315) »
    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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