Recombinant
RabMAb

Recombinant Anti-Nectin 2 antibody [EPR6717] (Alexa Fluor® 647) (ab237120)

Overview

  • Product name

    Anti-Nectin 2 antibody [EPR6717] (Alexa Fluor® 647)
    See all Nectin 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6717] to Nectin 2 (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Nectin 2 aa 500-600 (intracellular). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HT-29 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Dissociation constant (KD)

    KD = 5.30 x 10 -7 M
    Learn more about KD
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6717
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in HT-29 fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function

    Probable cell adhesion protein.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 112 antibody
    • CD112 antibody
    • CD112 antigen antibody
    • Herpes virus entry mediator B antibody
    • Herpes virus entry protein B antibody
    • Herpesvirus entry mediator B antibody
    • Herpesvirus entry protein B antibody
    • Hve B antibody
    • HveB antibody
    • Nectin-2 antibody
    • Nectin2 antibody
    • Poliovirus receptor like 2 antibody
    • Poliovirus receptor related 2 (herpesvirus entry mediator B) antibody
    • Poliovirus receptor related 2 antibody
    • Poliovirus receptor related protein 2 antibody
    • Poliovirus receptor-related protein 2 antibody
    • PRR 2 antibody
    • PRR2 antibody
    • PVRL 2 antibody
    • PVRL2 antibody
    • PVRL2_HUMAN antibody
    • PVRR 2 antibody
    • PVRR2 antibody
    see all

Images

  • ab237120 staining Nectin 2 in HT-29 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237120 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HT-29 cells fixed with 4% formaldehyde (10 min).

References

ab237120 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab237120.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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