Anti-Nectin 2 antibody - N-terminal (ab154895)
Key features and details
- Rabbit polyclonal to Nectin 2 - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nectin 2 antibody - N-terminal
See all Nectin 2 primary antibodies -
Description
Rabbit polyclonal to Nectin 2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey
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Immunogen
Recombinant fragment, corresponding to a region within N terminal amino acids 1-239 of Human Nectin 2 (Q92692).
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Positive control
- Human gastric carcinoma tissue. NT2D1, IMR32 and MCF-7 whole cell lysate.
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General notes
Keep as concentrated solution.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 58 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used |
Target
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Function
Probable cell adhesion protein. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5819 Human
- Omim: 600798 Human
- SwissProt: Q92692 Human
- Unigene: 655455 Human
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Alternative names
- CD 112 antibody
- CD112 antibody
- CD112 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody - N-terminal (ab154895)Immunohistochemical analysis of paraffin-embedded gastric carcinoma labeling Nectin 2 with ab154895 at 1/500 dilution.
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Western blot - Anti-Nectin 2 antibody - N-terminal (ab154895)Anti-Nectin 2 antibody - N-terminal (ab154895) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 58 kDa
10% SDS PAGE
Protocols
References (2)
ab154895 has been referenced in 2 publications.
- Kobayashi H et al. Aeromonas sobria serine protease decreases epithelial barrier function in T84 cells and accelerates bacterial translocation across the T84 monolayer in vitro. PLoS One 14:e0221344 (2019). PubMed: 31419250
- Brandetti E et al. MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma. Oncoimmunology 6:e1316439 (2017). PubMed: 28680748
