Anti-Nectin 2 antibody - N-terminal (ab154895)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Nectin 2 antibody - N-terminal
See all Nectin 2 primary antibodies -
Description
Rabbit polyclonal to Nectin 2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey
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Immunogen
Recombinant fragment, corresponding to a region within N terminal amino acids 1-239 of Human Nectin 2 (Q92692).
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Positive control
- Human gastric carcinoma tissue. NT2D1, IMR32 and MCF-7 whole cell lysate.
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General notes
Keep as concentrated solution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 58 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used |
Target
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Function
Probable cell adhesion protein. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5819 Human
- Omim: 600798 Human
- SwissProt: Q92692 Human
- Unigene: 655455 Human
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Alternative names
- CD 112 antibody
- CD112 antibody
- CD112 antigen antibody
see all
Images
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Western blot - Anti-Nectin 2 antibody - N-terminal (ab154895)Anti-Nectin 2 antibody - N-terminal (ab154895) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 58 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody - N-terminal (ab154895)Immunohistochemical analysis of paraffin-embedded gastric carcinoma labeling Nectin 2 with ab154895 at 1/500 dilution.
References
This product has been referenced in:
- Brandetti E et al. MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma. Oncoimmunology 6:e1316439 (2017). Read more (PubMed: 28680748) »
