Overview

  • Product name
    Anti-Nectin 2 antibody - N-terminal
    See all Nectin 2 primary antibodies
  • Description
    Rabbit polyclonal to Nectin 2 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Monkey
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 1-239 of Human Nectin 2 (Q92692).

  • Positive control
    • Human gastric carcinoma tissue. NT2D1, IMR32 and MCF-7 whole cell lysate.
  • General notes
    Keep as concentrated solution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used

Target

  • Function
    Probable cell adhesion protein.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • CD 112 antibody
      • CD112 antibody
      • CD112 antigen antibody
      • Herpes virus entry mediator B antibody
      • Herpes virus entry protein B antibody
      • Herpesvirus entry mediator B antibody
      • Herpesvirus entry protein B antibody
      • Hve B antibody
      • HveB antibody
      • Nectin-2 antibody
      • Nectin2 antibody
      • Poliovirus receptor like 2 antibody
      • Poliovirus receptor related 2 (herpesvirus entry mediator B) antibody
      • Poliovirus receptor related 2 antibody
      • Poliovirus receptor related protein 2 antibody
      • Poliovirus receptor-related protein 2 antibody
      • PRR 2 antibody
      • PRR2 antibody
      • PVRL 2 antibody
      • PVRL2 antibody
      • PVRL2_HUMAN antibody
      • PVRR 2 antibody
      • PVRR2 antibody
      see all

    Images

    • Western blot - Anti-Nectin 2 antibody - N-terminal (ab154895)
      Western blot - Anti-Nectin 2 antibody - N-terminal (ab154895)
      Anti-Nectin 2 antibody - N-terminal (ab154895) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

      Predicted band size: 58 kDa



      10% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody - N-terminal (ab154895)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody - N-terminal (ab154895)
      Immunohistochemical analysis of paraffin-embedded gastric carcinoma labeling Nectin 2 with ab154895 at 1/500 dilution.

    References

    This product has been referenced in:
    • Brandetti E  et al. MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma. Oncoimmunology 6:e1316439 (2017). Read more (PubMed: 28680748) »

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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