Overview

  • Product name

    Anti-Nectin 3 antibody [103-A1]
    See all Nectin 3 primary antibodies
  • Description

    Rat monoclonal [103-A1] to Nectin 3
  • Host species

    Rat
  • Tested applications

    Suitable for: Functional Studies, ICC/IF, Flow Cyt, IHC-Fr, IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to Mouse Nectin 3 extracellular domain, amino acids 50-400 fused to IgG Fc.

  • Positive control

    • L-cells (A cultured line of C3H mouse fibroblasts)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Provided as a 0.2µm purified antibody solution
  • Clonality

    Monoclonal
  • Clone number

    103-A1
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.
    • Tissue specificity

      Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.
    • Sequence similarities

      Belongs to the nectin family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD113 antibody
      • CDw113 antibody
      • DKFZP566B0846 antibody
      • FLJ90624 antibody
      • Nectin-3 antibody
      • Nectin3 antibody
      • Poliovirus receptor related 3 antibody
      • Poliovirus receptor related protein 3 antibody
      • Poliovirus receptor-like 3 antibody
      • Poliovirus receptor-related protein 3 antibody
      • PPR3 antibody
      • PRR3 antibody
      • Pvrl3 antibody
      • PVRL3_HUMAN antibody
      • PVRR3 antibody
      see all

    References

    This product has been referenced in:

    • Lachke SA  et al. The cell adhesion gene PVRL3 is associated with congenital ocular defects. Hum Genet 131:235-50 (2012). IHC-Fr ; Mouse . Read more (PubMed: 21769484) »
    • Lin YW  et al. Localization of ubiquitin specific protease 26 at blood-testis barrier and near Sertoli cell-germ cell interface in mouse testes. Int J Androl 34:e368-77 (2011). Read more (PubMed: 21449984) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Answer

    Thank you for contacting Abcam.

    According to our notes, ab16913 is not suitable for western blotting. However the Nectin3 antibodies listed below, are suitable for use in western blotting:

    https://www.abcam.com/Nectin-3-antibody-ab63931.html
    https://www.abcam.com/Nectin-3-antibody-ab86098.html
    https://www.abcam.com/Nectin-3-antibody-ab98342.html



    If there is anything else I can help you with, then please let me know.

    Read More
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Embryonic stem cells)
    Specification
    Embryonic stem cells
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted May 10 2011

    Application
    Flow Cytometry
    Sample
    Mouse Cell (Embryonic stem cells)
    Specification
    Embryonic stem cells
    Preparation
    Cell harvesting/tissue preparation method: Trypsination
    Sample buffer: PBS, 4%FCS
    Permeabilization
    No
    Gating Strategy
    Debris was gated away by FSC/SSC and dead cells by PI.

    Abcam user community

    Verified customer

    Submitted May 09 2011

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (testis)
    Specification
    testis
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

    Y Toyama

    Verified customer

    Submitted Aug 25 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up