Overview

  • Product name

  • Description

    Rabbit polyclonal to Nectin 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to a region surrounding amino acid 536 of mouse nectin 3

  • Positive control

    • 3T3 cell lysate; Rat kidney lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab63931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. See Abreview.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).

Target

  • Function

    Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.
  • Tissue specificity

    Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.
  • Sequence similarities

    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD113 antibody
    • CDw113 antibody
    • DKFZP566B0846 antibody
    • FLJ90624 antibody
    • Nectin-3 antibody
    • Nectin3 antibody
    • Poliovirus receptor related 3 antibody
    • Poliovirus receptor related protein 3 antibody
    • Poliovirus receptor-like 3 antibody
    • Poliovirus receptor-related protein 3 antibody
    • PPR3 antibody
    • PRR3 antibody
    • Pvrl3 antibody
    • PVRL3_HUMAN antibody
    • PVRR3 antibody
    see all

Images

  • All lanes : Anti-Nectin 3 antibody (ab63931) at 1 µg

    Lane 1 : Rat kidney lysate
    Lane 2 : 3T3 cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

References

This product has been referenced in:

  • Li J  et al. Chronic mild corticosterone exposure during adolescence enhances behaviors and upregulates neuroplasticity-related proteins in rat hippocampus. Prog Neuropsychopharmacol Biol Psychiatry 89:400-411 (2019). Read more (PubMed: 30392783) »
  • Gong Q  et al. Chronic Stress Reduces Nectin-1 mRNA Levels and Disrupts Dendritic Spine Plasticity in the Adult Mouse Perirhinal Cortex. Front Cell Neurosci 12:67 (2018). Read more (PubMed: 29593501) »
See all 15 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

We have previously been in contact regarding ab63931, which was not providing satisfactory results.

We provided some troubleshooting tips for the protocol, and I am writing to inquire if these suggestions have helped to improve the results? I would be pleased to receive an update on how these experiments are progressing.

If the antibody is still not working as stated on the datasheet, and the item was purchased within 6 months of contacting us, we would be happy to replace or refund the antibody.

I wish you the best of luck with your research and I look forward to hearing from you. Please do not hesitate to contact us if you have any further questions.



**

Our tips:

Thank you for your reply.

What I wanted to say is that the HEK293 cells do also have Nectin-3 expression. The antibody ab63931 is likely to recognize also the Nectin-3 isoforms expressed by the human cell. Therefore it could be that the observed band is not the transfected Nectin-3 gamma but the Nectin-3 of the HEK293 cell.
In order to exclude this, it would be necessary to test the HEK293 cells alone or mock transfected. Have you done this? If so, do you observe a band at the same molecular weight in the HEK293 alone/mock transfected?

I am looking forward to your reply. Please do not hesitate to call us as well (01.46.94.62.96) if you have any question.

Read More

Answer

Thank you for your reply.

What I wanted to say is that the HEK293 cells do also have Nectin-3 expression. The antibody ab63931 is likely to recognize also the Nectin-3 isoforms expressed by the human cell. Therefore it could be that the observed band is not the transfected Nectin-3 gamma but the Nectin-3 of the HEK293 cell.
In order to exclude this, it would be necessary to test the HEK293 cells alone or mock transfected. Have you done this? If so, do you observe a band at the same molecular weight in the HEK293 alone/mock transfected?

I am looking forward to your reply. Please do not hesitate to call us as well if you have any question.

Read More

Answer

Thank you for providing these additional details and your patience.

The laboratory has confirmed that the immunogen for thisantibody isa synthetic peptide corresponding to residues surrounding amino acids 536 of mouse Nectin-3 and that this region isindeed lacking inNectin-3 gamma.Howeverthe HEK293 are human and this immunogen has 99-100% with human Nectin-3 alpha. Could it be therefore that the detected band corresponds to thehuman form from the HEK293?Do you have a band in the untransfected cells as well?

Can you please send us an image from the obtainedresults as well?

Thank you very much for your cooperation. We will try our best to resolve this case. Should the antibody you received be faulty, we will replace or refund it for you.

I am looking forward to receive your reply.

Read More

Question
Answer

Thank you for your patience.

Unfortunately we can not provide the exact immunogen sequence for this antibody as it is proprietary information of the laboratory.

As said, please provide us with more information about the experiment. We and the laboratory would be happy to help. Please do not forget to mention in the questionnaire also what kind of cells were transfected and what was the species that the protein was derived from.

I am looking forward to receive the completed questionnaire from you in order to be able to investigate this issue further.

Thank you for your cooperation.

Read More

Answer

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I have contacted the laboratory requesting further details of the immunogen sequence.

As we discussed on the phone, I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

Read More
Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole C57BL/6 mouse hippocampus)
Loading amount
40 µg
Specification
Whole C57BL/6 mouse hippocampus
Gel Running Conditions
Non-reduced Denaturing (NuPAGE® 10% Bis-Tris gel (Invitrogen))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 30 2009

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Hippocampus)
Specification
Hippocampus
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Dec 17 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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