Overview

  • Product name
  • Description
    Rabbit polyclonal to PVRL4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 29-300 of Human PVRL4 (Q96NY8).

  • Positive control
    • 293T, A431, Jurkat and Raji whole cell lysates, MCF7 xenograft

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.01% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1. Does not act as receptor for alpha-herpesvirus entry into cells.
  • Tissue specificity
    Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.
  • Involvement in disease
    Defects in PVRL4 are the cause of ectodermal dysplasia-syndactyly syndrome type 1 (EDSS1) [MIM:613573]. EDSS1 is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDSS1 is characterized by the association of hair and teeth abnormalities with cutaneous syndactyly of the hands and/or feet. Hair morphologic abnormalities include twists at irregular intervals (pilli torti) and swelling along the shafts, particularly associated with areas of breakage. Dental findings consist of abnormally widely spaced teeth, with peg-shaped and conical crowns. Patients have normal sweating.
  • Sequence similarities
    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE.
  • Cellular localization
    Cell membrane. Cell junction > adherens junction. Colocalizes with MLLT4 at cadherin-based adherens junctions and Secreted. The secreted form is found in breast tumor patients.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686K05193 antibody
    • EDSS1 antibody
    • Ig superfamily receptor LNIR antibody
    • Nectin 4 antibody
    • Nectin-4 antibody
    • poliovirus receptor-related 4 antibody
    • Processed poliovirus receptor-related protein 4 antibody
    • PRR4 antibody
    • pvrl4 antibody
    • PVRL4_HUMAN antibody
    see all

Images

  • Anti-Nectin-4 antibody (ab155692) at 1/1000 dilution + Raji whole cell
    lysate at 30 µg

    Predicted band size: 55 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded MCF7 xenograft labeling PVRL4 with ab155692 antibody at 1/500.

References

ab155692 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 37°C
Sample
Human Cell (recombinat human protein expressed in CHO cells)
Specification
recombinat human protein expressed in CHO cells
Permeabilization
No
Fixative
Paraformaldehyde
Username

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Submitted Dec 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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