Key features and details
- Rabbit polyclonal to Nectin-4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Nectin-4 antibody
See all Nectin-4 primary antibodies
DescriptionRabbit polyclonal to Nectin-4
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: CowDoes not react with: Mouse
Recombinant fragment corresponding to Human Nectin-4 aa 91-199.
Database link: Q96NY8
- WB: Raji and NIH/3T3 whole cell lysate. IHC-P: Human pancreatic cancer tissue. ICC/IF: MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500.|
|WB||1/500 - 1/5000.|
|ICC/IF||1/50 - 1/200.|
FunctionSeems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1. Does not act as receptor for alpha-herpesvirus entry into cells.
Tissue specificityPredominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.
Involvement in diseaseDefects in PVRL4 are the cause of ectodermal dysplasia-syndactyly syndrome type 1 (EDSS1) [MIM:613573]. EDSS1 is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDSS1 is characterized by the association of hair and teeth abnormalities with cutaneous syndactyly of the hands and/or feet. Hair morphologic abnormalities include twists at irregular intervals (pilli torti) and swelling along the shafts, particularly associated with areas of breakage. Dental findings consist of abnormally widely spaced teeth, with peg-shaped and conical crowns. Patients have normal sweating.
Sequence similaritiesBelongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsThe soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE.
Cellular localizationCell membrane. Cell junction > adherens junction. Colocalizes with MLLT4 at cadherin-based adherens junctions and Secreted. The secreted form is found in breast tumor patients.
- Information by UniProt
- DKFZp686K05193 antibody
- EDSS1 antibody
- Ig superfamily receptor LNIR antibody
All lanes : Anti-Nectin-4 antibody (ab235897) at 1/500 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Paraffin-embedded human pancreatic cancer tissue stained for Nectin-4 using ab235897 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for Nectin-4 using ab235897 at 1/133 dilution in ICC/IF. The cells were blocked in 10% normal goat serum and incubated with primary antibody at 4°C overnight. Secondary antibody was Aleza Fluor® 488-conjugated goat anti-rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235897 has not yet been referenced specifically in any publications.