Overview

  • Product name

  • Description

    Rabbit polyclonal to Nectin-4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: CowDoes not react with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human Nectin-4 aa 91-199.
    Database link: Q96NY8

  • Positive control

    • WB: Raji and NIH/3T3 whole cell lysate. IHC-P: Human pancreatic cancer tissue. ICC/IF: MCF7 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab235897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500.
WB 1/500 - 1/5000.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1. Does not act as receptor for alpha-herpesvirus entry into cells.
  • Tissue specificity

    Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.
  • Involvement in disease

    Defects in PVRL4 are the cause of ectodermal dysplasia-syndactyly syndrome type 1 (EDSS1) [MIM:613573]. EDSS1 is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDSS1 is characterized by the association of hair and teeth abnormalities with cutaneous syndactyly of the hands and/or feet. Hair morphologic abnormalities include twists at irregular intervals (pilli torti) and swelling along the shafts, particularly associated with areas of breakage. Dental findings consist of abnormally widely spaced teeth, with peg-shaped and conical crowns. Patients have normal sweating.
  • Sequence similarities

    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE.
  • Cellular localization

    Cell membrane. Cell junction > adherens junction. Colocalizes with MLLT4 at cadherin-based adherens junctions and Secreted. The secreted form is found in breast tumor patients.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686K05193 antibody
    • EDSS1 antibody
    • Ig superfamily receptor LNIR antibody
    • Nectin 4 antibody
    • Nectin-4 antibody
    • poliovirus receptor-related 4 antibody
    • Processed poliovirus receptor-related protein 4 antibody
    • PRR4 antibody
    • pvrl4 antibody
    • PVRL4_HUMAN antibody
    see all

Images

  • All lanes : Anti-Nectin-4 antibody (ab235897) at 1/500 dilution

    Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
  • Paraffin-embedded human pancreatic cancer tissue stained for Nectin-4 using ab235897 at 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.

  • 4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for Nectin-4 using ab235897 at 1/133 dilution in ICC/IF. The cells were blocked in 10% normal goat serum and incubated with primary antibody at 4°C overnight. Secondary antibody was Aleza Fluor® 488-conjugated goat anti-rabbit IgG (H+L).

References

ab235897 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab235897.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up