Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15613-68] to Nectin-4
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-Nectin-4 antibody [EPR15613-68]
See all Nectin-4 primary antibodies
DescriptionRabbit monoclonal [EPR15613-68] to Nectin-4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Nectin-4 aa 50-200. The exact sequence is proprietary.
Database link: Q96NY8
- T47D, MCF7 and Human placenta lysates; Human placenta tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab192033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 44, 55, 66 kDa (predicted molecular weight: 55 kDa).|
|IHC-P||1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionSeems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1. Does not act as receptor for alpha-herpesvirus entry into cells.
Tissue specificityPredominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.
Involvement in diseaseDefects in PVRL4 are the cause of ectodermal dysplasia-syndactyly syndrome type 1 (EDSS1) [MIM:613573]. EDSS1 is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDSS1 is characterized by the association of hair and teeth abnormalities with cutaneous syndactyly of the hands and/or feet. Hair morphologic abnormalities include twists at irregular intervals (pilli torti) and swelling along the shafts, particularly associated with areas of breakage. Dental findings consist of abnormally widely spaced teeth, with peg-shaped and conical crowns. Patients have normal sweating.
Sequence similaritiesBelongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsThe soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE.
Cellular localizationCell membrane. Cell junction > adherens junction. Colocalizes with MLLT4 at cadherin-based adherens junctions and Secreted. The secreted form is found in breast tumor patients.
- Information by UniProt
- DKFZp686K05193 antibody
- EDSS1 antibody
- Ig superfamily receptor LNIR antibody
All lanes : Anti-Nectin-4 antibody [EPR15613-68] (ab192033) at 1/1000 dilution
Lane 1 : T47D lysate
Lane 2 : MCF7 lysate
Lane 3 : Human placenta lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Nectin-4 with ab192033 at 1/3000 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Inset: Negative control: using PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ab192033 has been referenced in 3 publications.
- Bekos C et al. NECTIN4 (PVRL4) as Putative Therapeutic Target for a Specific Subtype of High Grade Serous Ovarian Cancer-An Integrative Multi-Omics Approach. Cancers (Basel) 11:N/A (2019). PubMed: 31137558
- Zhang Y et al. High expression of Nectin-4 is associated with unfavorable prognosis in gastric cancer. Oncol Lett 15:8789-8795 (2018). PubMed: 29805618
- Nishiwada S et al. Nectin-4 expression contributes to tumor proliferation, angiogenesis and patient prognosis in human pancreatic cancer. J Exp Clin Cancer Res 34:30 (2015). WB . PubMed: 25888293