Recombinant
RabMAb

Recombinant Anti-Nectin-4 antibody [EPR15613] (ab189514)

Overview

  • Product name

    Anti-Nectin-4 antibody [EPR15613]
    See all Nectin-4 primary antibodies
  • Description

    Rabbit monoclonal [EPR15613] to Nectin-4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Nectin-4 aa 50-200. The exact sequence is proprietary.
    Database link: Q96NY8

  • Positive control

    • T47D, MCF7 and Human placenta lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15613
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 44, 55, 66 kDa (predicted molecular weight: 55 kDa).

Target

  • Function

    Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1. Does not act as receptor for alpha-herpesvirus entry into cells.
  • Tissue specificity

    Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.
  • Involvement in disease

    Defects in PVRL4 are the cause of ectodermal dysplasia-syndactyly syndrome type 1 (EDSS1) [MIM:613573]. EDSS1 is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDSS1 is characterized by the association of hair and teeth abnormalities with cutaneous syndactyly of the hands and/or feet. Hair morphologic abnormalities include twists at irregular intervals (pilli torti) and swelling along the shafts, particularly associated with areas of breakage. Dental findings consist of abnormally widely spaced teeth, with peg-shaped and conical crowns. Patients have normal sweating.
  • Sequence similarities

    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE.
  • Cellular localization

    Cell membrane. Cell junction > adherens junction. Colocalizes with MLLT4 at cadherin-based adherens junctions and Secreted. The secreted form is found in breast tumor patients.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686K05193 antibody
    • EDSS1 antibody
    • Ig superfamily receptor LNIR antibody
    • Nectin 4 antibody
    • Nectin-4 antibody
    • poliovirus receptor-related 4 antibody
    • Processed poliovirus receptor-related protein 4 antibody
    • PRR4 antibody
    • pvrl4 antibody
    • PVRL4_HUMAN antibody
    see all

Images

  • All lanes : Anti-Nectin-4 antibody [EPR15613] (ab189514) at 1/1000 dilution

    Lane 1 : T47D cell lysate
    Lane 2 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 44 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 55 kDa (possible glycosylated form), 66 kDa (possible glycosylated form)

  • Anti-Nectin-4 antibody [EPR15613] (ab189514) at 1/1000 dilution + Human placenta lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 44,55 kDa why is the actual band size different from the predicted?
    Additional bands at: 66 kDa (possible glycosylated form)

References

ab189514 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189514.
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