Recombinant Anti-Nectin-4 antibody [EPR15613] - BSA and Azide free (ab250990)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15613] to Nectin-4 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Nectin-4 antibody [EPR15613] - BSA and Azide free
See all Nectin-4 primary antibodies -
Description
Rabbit monoclonal [EPR15613] to Nectin-4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250990 is the carrier-free version of ab189514.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15613 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 44, 55, 66 kDa (predicted molecular weight: 55 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 44, 55, 66 kDa (predicted molecular weight: 55 kDa). |
Target
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Function
Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1. Does not act as receptor for alpha-herpesvirus entry into cells. -
Tissue specificity
Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma. -
Involvement in disease
Defects in PVRL4 are the cause of ectodermal dysplasia-syndactyly syndrome type 1 (EDSS1) [MIM:613573]. EDSS1 is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDSS1 is characterized by the association of hair and teeth abnormalities with cutaneous syndactyly of the hands and/or feet. Hair morphologic abnormalities include twists at irregular intervals (pilli torti) and swelling along the shafts, particularly associated with areas of breakage. Dental findings consist of abnormally widely spaced teeth, with peg-shaped and conical crowns. Patients have normal sweating. -
Sequence similarities
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsThe soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE. -
Cellular localization
Cell membrane. Cell junction > adherens junction. Colocalizes with MLLT4 at cadherin-based adherens junctions and Secreted. The secreted form is found in breast tumor patients. - Information by UniProt
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Database links
- Entrez Gene: 81607 Human
- Omim: 609607 Human
- SwissProt: Q96NY8 Human
- Unigene: 492490 Human
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Alternative names
- DKFZp686K05193 antibody
- EDSS1 antibody
- Ig superfamily receptor LNIR antibody
see all
Images
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All lanes : Anti-Nectin-4 antibody [EPR15613] (ab189514) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : Human skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 40-70 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsThis data was developed using ab189514, the same antibody clone in a different buffer formulation.
ab189514 detected an 50kda nonspecific band in negative sample skeletal muscle while ab192033 didn't.
Blocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-Nectin-4 antibody [EPR15613] (ab189514) at 1/1000 dilution
Lane 1 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate
Lane 2 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Human placenta tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 40-70 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsThis data was developed using ab189514, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed in MDA-MB-231 is consistent with the literatures (PMID: 31617074, PMID: 24386110, PMID: 17474988).
Negative control: MDA-MB-231 (PMID: 31617074, PMID: 24386110, PMID: 17474988).
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All lanes : Anti-Nectin-4 antibody [EPR15613] (ab189514) at 1/1000 dilution
Lane 1 : T47D cell lysate
Lane 2 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 55 kDa
Additional bands at: 66 kDa (possible glycosylated form)This data was developed using ab189514, the same antibody clone in a different buffer formulation.
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Anti-Nectin-4 antibody [EPR15613] (ab189514) at 1/1000 dilution + Human placenta lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 44,55 kDa why is the actual band size different from the predicted?
Additional bands at: 66 kDa (possible glycosylated form)This data was developed using ab189514, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250990 has not yet been referenced specifically in any publications.