Key features and details
- Rabbit polyclonal to NEDD1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NEDD1 antibody
See all NEDD1 primary antibodies
DescriptionRabbit polyclonal to NEDD1
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Recombinant fragment corresponding to Human NEDD1 aa 411-660.
SPIRDDAVVNKGSDESIGKGDGFDFLPQLNSVFPPRKNPVTSSTSVLHSS PLNVFMGSPGKEENENRDLTAESKKIYMGKQESKDSFKQLAKLVTSGAES GNLNTSPSSNQTRNSEKFEKPENEIEAQLICEPPINGSSTPNPKIASSVT AGVASSLSEKIADSIGNNRQNAPLTSIQIRFIQNMIQETLDDFREACHRD IVNLQVEMIKQFHMQLNEMHSLLERYSVNEGLVAEIERLREENKRLRAHF
Database link: Q8NHV4
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
FunctionRequired for mitosis progression. Promotes the nucleation of microtubules from the spindle.
Sequence similaritiesContains 8 WD repeats.
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- FLJ35902 antibody
- GCP WD antibody
- NEDD-1 antibody
All lanes : Anti-NEDD1 antibody (ab230254) at 1/1000 dilution
Lane 1 : Mouse small intestine tissue lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
All lanes : Goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Observed band size: 72 kDa why is the actual band size different from the predicted?
Paraffin-embedded human glioma cancer tissue stained for NEDD1 using ab230254 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human thyroid tissue stained for NEDD1 using ab230254 at 1/100 dilution in immunohistochemical analysis.
NEDD1 was immunoprecipitated from 0.5 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 μg ab230254. Western blot was performed from the immunoprecipitate using ab230254. An HRP-conjugated light chain specific antibody was used as secondary antibody at 1/50,000 dilution.
Lane 1: Rabbit monoclonal IgG (1 μg) instead of ab230254 in HeLa whole cell lysate.
Lane 2: ab230254 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (Input).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230254 has not yet been referenced specifically in any publications.