Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab56317 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab56317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).
An additional band was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide and likely represents the mature form.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRequired for mitosis progression. Promotes the nucleation of microtubules from the spindle.
Sequence similaritiesContains 8 WD repeats.
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- FLJ35902 antibody
- GCP WD antibody
- NEDD-1 antibody
Anti-NEDD1 antibody (ab56317) at 0.1 µg/ml + HeLa cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
ab56317 has not yet been referenced specifically in any publications.