Anti-NEDD4-2 antibody (ab124643)
Key features and details
- Rabbit polyclonal to NEDD4-2
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NEDD4-2 antibody
See all NEDD4-2 primary antibodies -
Description
Rabbit polyclonal to NEDD4-2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to a region between residues 275 and 325 of Human NEDD4-2 (NP_001138439.1).
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Positive control
- Human prostate carcinoma; Mouse teratoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
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Notes |
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IHC-P
1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. |
Target
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Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. -
Tissue specificity
Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 4 WW domains. -
Post-translational
modificationsPhosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
Auto-ubiquitinated. -
Cellular localization
Cytoplasm. May be recruited to exosomes by NDFIP1. - Information by UniProt
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Database links
- Entrez Gene: 23327 Human
- Entrez Gene: 83814 Mouse
- Entrez Gene: 100172429 Orangutan
- Entrez Gene: 291553 Rat
- Omim: 606384 Human
- SwissProt: Q96PU5 Human
- SwissProt: Q8CFI0 Mouse
- SwissProt: Q5RBF2 Orangutan
see all -
Alternative names
- E3 ubiquitin protein ligase NEDD4 like protein antibody
- E3 ubiquitin-protein ligase NEDD4-like antibody
- KIAA0439 antibody
see all
Images
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ab124643, at 1/250 dilution, staining NEDD4-2 in formalin-fixed, paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry with DAB staining.
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ab124643, at 1/250 dilution, staining NEDD4-2 in formalin-fixed, paraffin-embedded Mouse teratoma tissue by Immunohistochemistry with DAB staining.
Datasheets and documents
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Datasheet download
References (0)
ab124643 has not yet been referenced specifically in any publications.